| 摘要: |
| 目的：研究健脾消癌方对HCT116/5-Fu耐药细胞增殖、凋亡、周期的影响。方法：采用5-Fu浓度递增间断刺激法诱导建立HCT116/5-Fu细胞，制备健脾消癌方含药血清，设立10%、15%、20%含药血清组，设立相同浓度无药血清对照组及10%胎牛血清组。采用CellTiter GLO法检测细胞增殖能力并计算细胞抑制率，采用PI-FACS法检测细胞周期变化，采用Annexin V-APC单染法检测细胞凋亡水平。结果：与对照组相比，不同浓度含药血清组对HCT116/5-Fu细胞增殖均有抑制作用，差异均有统计学意义（P＜0.05或P＜0.01），且随含药血清浓度的升高而抑制作用增强；与对照组相比，不同浓度含药血清组HCT116/5-Fu细胞凋亡率均明显提高，差异有统计学意义（P＜0.01），且随含药血清浓度升高细胞凋亡率提高幅度增大；与对照组相比，不同浓度含药血清组均可将细胞周期阻滞在G0/G1期，差异有统计学意义（P＜0.01）。结论：健脾消癌方可抑制HCT116/5-Fu细胞的增殖，促进HCT116/5-Fu细胞的凋亡，阻滞其细胞周期进程，且其作用随浓度的升高而明显增强，具有改善结直肠癌耐药的作用。 |
| 关键词: 健脾消癌方 HCT116/5-Fu细胞 增殖 凋亡 细胞周期 |
| DOI： |
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| Effect of Jianpi Xiaoai prescription on the proliferation，apoptosis，and cell cycle of colon cancer HCT116 cells resistant to 5-fluorouracil |
| KUANG Wanting,ZENG Puhua,NING Kangwen |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China;The Affiliated Hospital of Hunan Academy of Chinese Medicine，Changsha 410006，Hunan，China) |
| Abstract: |
| Objective：To investigate the effect of Jianpi Xiaoai prescription on the proliferation，apoptosis，and cell cycle of colon cancer HCT116 cells resistant to 5-fluorouracil (5-Fu). Methods：HCT116/5-Fu cells were established by intermittent stimulation with increasing concentrations of 5-Fu，and serum containing Jianpi Xiaoai prescription was prepared. The 10%，15%，and 20% serum containing Jianpi Xiaoai prescription groups were established，as well as non-drug serum control groups at the same concentrations and 10% fetal bovine serum group. CellTiter GLO was used to measure the proliferative capacity of cells and calculate cell inhibition rate; PI-FACS was used to observe the change in cell cycle; Annexin V-APC staining was used to measure the level of cell apoptosis. Results：Compared with the control groups，the serum containing Jianpi Xiaoai prescription groups at different concentrations showed a significant inhibitory effect on the proliferation of HCT116/5-Fu cells (P＜0.05 or P＜0.01)，and the inhibitory effect was enhanced with the increase in the concentration of serum containing Jianpi Xiaoai prescription. Compared with the control groups，the serum containing Jianpi Xiaoai prescription groups at different concentrations showed a significant increase in the apoptosis rate of HCT116/5-Fu cells (P＜0.01)，and the apoptosis rate increased with the increase in the concentration of serum containing Jianpi Xiaoai prescription. Compared with the control group，the serum containing Jianpi Xiaoai prescription groups at different concentrations had the cells arrested in the G0/G1 stage (P＜0.01). Conclusion：Jianpi Xiaoai prescription can inhibit the proliferation of HCT116/5-Fu cells，promote the apoptosis of HCT116/5-Fu cells，and induce cell cycle arrest，and such effect is significantly enhanced with the increase in concentration. Therefore，it can improve drug resistance in colorectal cancer. |
| Key words: Jianpi Xiaoai prescription HCT116/5-Fu cells proliferation apoptosis cell cycle |
