| 摘要: |
| 目的：观察艾灸对实验性类风湿性关节炎（RA）家兔炎症因子白细胞介素-10（IL-10）、白细胞介素-17（IL-17）和滑膜液中骨桥蛋白（OPN）、血管内皮细胞生长因子（VEGF）表达的影响,进一步探索艾灸治疗RA的效应机制。方法：选用CL级日本大耳白兔30只,随机分为空白组、RA模型组、治疗组,每组各10只。采用弗氏完全佐剂（FCA）法建立RA模型。造模后第7天,治疗组采用艾灸（悬灸）肾俞、足三里,每穴各灸5壮,每天1次,以6 d为1个疗程,共干预3个疗程；空白组、RA模型组不做任何干预。检测各组外周血IL-10、IL-17和滑膜液OPN、VEGF的含量,观察滑膜组织中OPN的表达。结果：RA模型组、治疗组IL-17、OPN及VEGF含量均较空白组明显升高（P<0.01）,IL-10明显降低（P<0.01）,治疗组各指标水平均优于RA模型组（P<0.01）。与空白组相比,RA模型组、治疗组滑膜组织OPN阳性表达增加（P<0.01）,治疗组OPN表达低于RA模型组（P<0.05）。结论：艾灸对实验性RA家兔滑膜炎症的抑制作用可能是通过调节OPN的表达,进而调节相关炎症因子及VEGF的表达,参与对RA滑膜炎症及血管翳形成的调节,其可能为艾灸治疗RA有效的内在机制。 |
| 关键词: 类风湿性关节炎 家兔 艾灸 OPN VEGF 实验研究 |
| DOI： |
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| Effect of moxibustion on inflammatory factors,osteopontin,and vascular endothelial growth factor in experimental rabbits with rheumatoid arthritis |
| REN Jigang,WANG Daxue,LEI Xiao |
| (Affiliated Hospital of Chuanbei Medical College,Nanchong 637000,Sichuan,China) |
| Abstract: |
| Objective：To investigate the effect of moxibustion on the inflammatory factors interleukin-10 (IL-10) and interleukin-17 (IL-17) and the expression of osteopontin (OPN) and vascular endothelial growth factor (VEGF) in synovial fluid in experimental rabbits with rheumatoid arthritis (RA).Methods：A total of 30 clean-class Japanese white rabbits were randomly divided into blank group,RA model group,and treatment group,with 10 rabbits in each group.Freund’s complete adjuvant was used to establish a model of RA.On day 7 after modeling,the rabbits in the treatment group were given moxibustion (suspended moxibustion) at Shenshu and Zusanli,with 5 moxa cones for each acupoint,once a day for 3 courses of treatment,and each course of treatment was 6 days；no intervention was given in the blank group and the RA model group.The levels of IL-10 and IL-17 in peripheral blood and the content of OPN and VEGF in synovial fluid were measured,and the expression of OPN in synovial tissue was observed.Results：Compared with the blank group,the RA model group and the treatment group had significant increases in the content of IL-17,OPN,and VEGF (P<0.01) and a significant reduction in IL-10 (P<0.01),and the treatment group had significantly better indices than the RA model group (P<0.01).Compared with the blank group,the RA model group and the treatment group had a significant increase in the expression of OPN in synovial tissue (P<0.01),and the treatment group had significantly lower expression of OPN than the RA model group (P<0.05).Conclusion：Moxibustion can inhibit synovitis in experimental rabbits with RA by regulating the expression of OPN,associated inflammatory cytokines,and VEGF,and it is involved in the regulation of synovitis and pannus formation in RA,which might be the mechanism of moxibustion in the effective treatment of RA. |
| Key words: rheumatoid arthritis rabbit moxibustion osteopontin vascular endothelial growth factor experimental study |
