| 摘要: |
| 目的：通过建立SD大鼠肌腱末端病模型，观察跟腱大体标本形态、组织学及碱性成纤维细胞生长因子（bFGF）表达量的变化，探讨推拿手法干预肌腱末端病的治疗作用及其作用机制。方法：将20只SD雄性大鼠喂养1周后，随机分成A、B、C、D 组，每组各5只。A组大鼠的右后肢为正常组（A1），左后肢为模型组（A2）；B、C、D组大鼠分为自然恢复组（B1、C1、D1）与治疗组（B2、C2、D2）。A1组不做任何处理，其余各组均采用跟腱注射Ⅰ型胶原酶的方法建立SD大鼠肌腱末端病模型。造模2周后对B2、C2、D2组进行推拿治疗，于患侧下肢施按揉手法，其余各组不做处理。A、B、C、D组分别在造模成功2周后及干预7、14、21 d后处死，对每组大鼠跟腱进行肉眼观察及病理组织形态学观察，并检测bFGF表达情况。结果：治疗组的损伤修复程度优于同期自然恢复组，且粘连程度比同期自然修复组轻。模型组bFGF含量升高，高于正常组（P＜0.01），随着损伤愈合，bFGF表达量逐渐下降，治疗组优于同期自然修复组，治疗后第14、21天最明显（P＜0.05或P＜0.01））。结论：推拿手法可下调bFGF表达量，促进受损组织愈合，防止肌腱粘连，对肌腱末端病起到干预作用。 |
| 关键词: 肌腱末端病 推拿 bFGF 实验研究 |
| DOI： |
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| Effect of massage manipulation on basic fibroblast growth factor in rats with enthesis |
| WEI Qingbo,GU Jialing,ZHANG Feng |
| (School of Acupuncture and Massage，Nanjing University of Chinese Medicine，Nanjing 210023，Jiangsu，China;Hanlin College，Nanjing University of Chinese Medicine，Taizhou 225300，Jiangsu，China) |
| Abstract: |
| Objective：To investigate the changes in the morphology and histology of the gross specimen of calcaneal tendon and the expression level of basic fibroblast growth factor (bFGF) in Sprague-Dawley rats withenthesis，as well as the therapeutic effect of massage manipulation on enthesis and its mechanism of action.Methods：After 1 week of feeding，20 male Sprague-Dawley rats were randomly divided into groups A，B，C，and D，with 5 rats in each group.The right hindlimbs of the rats in group A were selected as normal group (A1)，and the left hindlimbs were selected as model group (A2).Groups B，C，and D were each divided into natural recovery group (B1，C1，and D1) and treatment groups (B2，C2，and D2).Group A1 was not given any treatment，and the other groups were given the injection ofcollagenase type 1 via the calcaneal tendon to establish a Sprague-Dawley rat model of enthesis.After 2 weeks of modeling，massage was performed for groups B2，C2，and D2，with foulage manipulation for the ipsilateral lower limb，and no treatment was given for the other groups.The rats in groups A，B，C，and D were sacrificed after 2 weeks of successful modeling and 7，14，and 21 days of intervention；the calcaneal tendon was observed with the naked eye，and pathological and histomorphological observations were also performed；the expression of bFGF was measured.Results：Compared with the natural recovery group at the corresponding period of time，the treatment group had a significantly better degree ofdamage repair and a significantly lower degree of adhesion.Compared with the normal group，the model group had a significant increase in the content ofbFGF (P＜0.01)，and the expression level of bFGF gradually decreasedwith the healing of injury；thetreatment group was superior to the natural recovery group at the corresponding period of time，with the most significant effect on days 14 and 21 after treatment (P＜0.05 or P＜0.01).Conclusion：Massage manipulation can downregulate the expression level of bFGF，promote the healing of injured tissue，preventtendon adhesion，and thus exert atherapeutic effect on enthesis. |
| Key words: enthesis massage basic fibroblast growth factor experimental study |
