| 摘要: |
| 目的：采用高尿酸血症模型大鼠研究抑酸复合茶对高尿酸血症的防治作用,并探讨其降尿酸的作用机制。方法：大鼠适应性饲养1周后根据随机分组原则分为正常对照组、模型组、别嘌醇组及抑酸复合茶高、低剂量组,每组各12只。除正常对照组外,其余各组均采用腺嘌呤联合氧嗪酸钾连续灌胃3周以建立高尿酸血症模型,建模同时给予药物干预,观察各组大鼠给药前后血尿酸（UA）及给药后尿素氮（BUN）、肌酐（Scr）、胱抑素C（CysC）、一氧化氮（NO）的变化。采用Western blot法检测抑酸复合茶对HEK293细胞URAT1和GLUT9表达的影响。结果：给药后,与模型组比较,抑酸复合茶高剂量组UA、BUN、Scr、CysC均降低,NO升高,差异均有统计学意义（P<0.05）;低剂量组UA、BUN降低,NO升高,差异亦有统计学意义（P<0.05）;与别嘌醇组比较,抑酸复合茶高剂量组CysC下降,NO升高,差异有统计学意义（P<0.05）;抑酸复合茶还可使HEK293细胞中URAT1和GLUT9的表达量降低。结论：抑酸复合茶对高尿酸血症大鼠降尿酸作用明显,且对血管内皮细胞和肾功能具有一定的保护作用;其作用机制可能是通过下调肾脏URAT1和GLUT9蛋白表达而发挥。 |
| 关键词: 高尿酸血症 大鼠 抑酸复合茶 实验研究 |
| DOI： |
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| Effect of Yi-Suan-Cha on renal function and its mechanism of action in reducing uric acid in a rat model of hyperuricemia |
| TAO Hui,QIN Yuanyuan,MENG Jianjun |
| (The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi,China) |
| Abstract: |
| Objective：To investigate the role of Yi-Suan-Cha in the prevention and treatment of hyperuricemia and its mechanism of action in reducing uric acid (UA) in a rat model of hyperuricemia.Methods：After one week of adaptive feeding,the rats were randomly divided into normal control group,model group,allopurinol group,and high- and low-dose Yi-Suan-Cha groups,with 12 rats in each group.All rats except those in the normal control group were given adenine combined with oteracil potassium by gavage for 3 consecutive weeks to establish a model of hyperuricemia,and drug intervention was given at the same time of modeling.Blood UA,blood urea nitrogen (BUN),serum creatinine (Scr),cystatin C (CysC),and nitric oxide (NO) were observed before and after administration.Western blot was used to evaluate the effect of Yi-Suan-Cha on the expression of URAT1 and GLUT9 in HEK293 cells.Results：Compared with the model group after administration,the high-dose Yi-Suan-Cha group had a significant reduction in UA,BUN,Scr,and CysC and a significant increase in NO (P<0.05),and the low-dose Yi-suan-Cha group had a significant reduction in UA and BUN and a significant increase in NO (P<0.05).Compared with the allopurinol group,the high-dose Yi-Suan-Cha group had a significant reduction in CysC and a significant increase in NO (P<0.05).Yi-Suan-Cha reduced the expression of URAT1 and GLUT9.Conclusion：Yi-Suan-Cha has a marked effect in reducing UA in rats with hyperuricemia and can exert a certain protective effect on vascular endothelial cells and renal function,possibly by downregulating the protein expression of URAT1 and GLUT9 in the kidney. |
| Key words: hyperuricemia rat Yi-Suan-Cha experimental study |
