| 摘要: |
| 目的：探讨内异方对大鼠子宫内膜异位症细胞间黏附分子1（ICAM-1）的影响。方法：采用自体子宫内膜移植法制备大鼠子宫内膜异位症模型，将其分为内异方高、中、低剂量组（予内异方药液干预，给药剂量分别为15 g/kg、7.5 g/kg、3.75 g/kg），阳性组（予达那唑胶囊药液干预，给药剂量为36 mg/kg），模型组和假手术组（均灌胃等体积溶媒），灌胃4 w。采用光镜观察异位内膜的病理组织变化，并评价病变分级，采用免疫组化法检测异位内膜组织的ICAM-1阳性表达。结果：模型组大鼠异位内膜体积大，腺体数量较多，间质层丰富，有大量炎性细胞浸润；内异方各剂量组大鼠的异位内膜有不同程度的萎缩，体积均较模型组显著缩小，间质炎性细胞数量较少。与模型组相比，内异方各剂量组病变分级程度均明显减轻，异位内膜组织的ICAM-1表达均明显降低，差异有统计学意义（P＜ 0.01）。结论：内异方具有治疗子宫内膜异位症的作用，其作用机制可能与抑制异位子宫内膜在盆腹腔处的黏附等有关。 |
| 关键词: 子宫内膜异位症 内异方 细胞间黏附分子-1 实验研究 |
| DOI： |
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| Effect of Neiyi prescription on intercellular adhesion molecule-1 in rats with endometriosis |
| QIAN Xiufang,SUN Huixian |
| (Beijing Armed Police Corps Hospital，Beijing 100027，China) |
| Abstract: |
| Objective：To investigate the effect of Neiyi prescription on intercellular adhesion molecule-1 (ICAM-1) in rats with endometriosis.Methods：A rat model of endometriosis was established by autologous endometrial transplantation，and then the rats were divided into high-，middle-，and low-dose Neiyi prescription groups (treated with the solution of Neiyi prescription at a dose of 15 g/kg，7.5 g/kg，and 3.75 g/kg，respectively)，positive group (treated with the solution of danazol capsules at a dose of 36 mg/kg)，model group (treated with an equal volume of solvent by gavage)，and sham-operation group (treated with an equal volume of solvent by gavage)；the course of treatment was 4 weeks by gavage.Histopathological changes of the ectopic endometrium were observed under a light microscope，and the grade of lesions was determined；immunohistochemistry was used to measure the positive expression of ICAM-1 in the ectopic endometrium.Results：The rats in the model group had a large volume of the ectopic endometrium，a large number of glands，abundant mesenchymes，and massive inflammatory cell infiltration，and those in the high-，middle-，and low-dose Neiyi prescription groups had varying degrees of atrophy of the ectopic endometrium and significant reductions in the volume of the ectopic endometrium and the number of inflammatory cells compared with the model group.Compared with the model group，the high-，middle-，and low-dose Neiyi prescription groups had significant reductions in the pathological grade of lesions and the expression of ICAM-1 in the ectopic endometrium (P＜0.01).Conclusion：Neiyi prescription can exert a therapeutic effect on endometriosis，possibly by inhibiting the adhesion of the ectopic endometrium in the pelvic and abdominal cavity. |
| Key words: endometriosis Neiyi prescription intercellular adhesion molecule-1 experimental study |
