| 摘要: |
| 目的：筛选并准确测定地黄活血汤调控白细胞介素（IL）-6表达治疗子宫平滑肌瘤的活性成分，作为其基于功效的质量评价指标。方法：活性筛选采用酶联免疫吸附（ELISA）法，分别加入地黄活血汤中所含各成分单体，测定其对IL-6表达的影响，将产生显著影响者列入候选；以上述候选成分为指标，进行高效液相色谱法（HPLC）含量测定研究，使用Waters Xbridge C18色谱柱（4.6 mm×250 mm，5 μm），乙腈-0.2%磷酸为流动相，梯度洗脱，流速1.0 ml/min，检测波长254 nm，柱温30℃，进样量10 μl；选择毛蕊花糖苷、木通皂苷D为指标性成分，共测定10批药材制备的供试品溶液。结果：ELISA法检测出毛蕊花糖苷、木通皂苷D、川续断皂苷乙、表儿茶素、芍药苷具有较为显著的IL-6表达调控活性；含量测定结果表明，毛蕊花糖苷、木通皂苷D在地黄活血汤中含量分别为0.0370～0.0534 mg/ml和0.0419～0.0590 mg/ml。结论：地黄活血汤中的毛蕊花糖苷、木通皂苷D能体外抑制IL-6的表达，建议列为该方基于功效主治的质量控制指标成分。 |
| 关键词: 地黄活血汤 子宫平滑肌瘤 IL-6 毛蕊花糖苷 木通皂苷D 实验研究 |
| DOI： |
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| Screening and determination of the active components of Dihuang Huoxue decoction for the treatment of leiomyoma of the uterus by regulating the expression of interleukin-6 |
| ZENG Xiufang,TAN Meiying |
| (The Ninth People’s Hospital of Nanhai District，Foshan 528203，Guangdong，China;Guangdong Second Provincial Traditional Chinese Medicine Hospital & Guangdong Research Institute of Traditional Chinese Medicine Manufacturing Technology，Guangzhou 510095，Guangdong，China) |
| Abstract: |
| Objective：To screen out and measure the active components of Dihuang Huoxue decoction for the treatment of leiomyoma of uterus by regulating the expression of interleukin-6 (IL-6)，as function-based quality assessment indices.Methods：ELISA was used to screen out the active components，and each component of Dihuang Huoxue decoction was added to observe their influence on the expression of IL-6，among which the components with marked influence were included as candidates.To measure the content of these candidate components，high-performance liquid chromatography was performed on a Waters Xbridge C18 column (4.6 mm×250 mm，5 μm) with a mobile phase of acetonitrile-0.2% phosphoric acid for gradient elution，at a flow rate of 1.0 ml/min，a detection wavelength of 254 nm，a column temperature of 30℃，and a sample size of 10 μl.Verbascoside and akebia saponin D were selected as index components，and the test article solutions of 10 batches of medicinal material were measured.Results：ELISA showed that verbascoside，akebia saponin D，dipsacoside B，epicatechin，and paeoniflorin had a marked effect in regulating the expression of IL-6.The results of content determination showed that the content of verbascoside and akebia saponin D in Dihuang Huoxue decoction were 0.0370～0.0534 mg/mL and 0.0419～0.0590 mg/mL，respectively.Conclusion：Verbascoside and akebia saponin D in Dihuang Huoxue decoction can inhibit the expression of IL-6 in vitro and are recommended to be included as function-based quality assessment indices. |
| Key words: Dihuang Huoxue decoction leiomyoma of the uterus interleukin-6 verbascoside akebia saponin D experimental study |
