| 摘要: |
| 目的:建立筋骨痛巴布膏的质量标准。方法：筛选方中独活、大黄、苏木及冰片进行薄层色谱鉴别；HPLC法对成品中大黄素进行含量测定。结果：制定了巴布膏中独活等药材的薄层鉴别方法；以大黄素为指标，以Kromasil C18色谱柱（250 mm×4.6 mm，5 μm）为固定相，甲醇-0.1%磷酸溶液（85∶15）为流动相；检测波长254 nm条件下，成品中大黄素能有效分离，方法学结果可行。结论:该法方便准确、灵敏度高、重现性好，能够为筋骨痛巴布膏质量标准的制订提供客观精准的依据。 |
| 关键词: 内病外治 筋骨痛巴布膏 质量标准 薄层色谱法 |
| DOI： |
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| Quality standard for cataplasma for bone and muscle pain developed by the method of external treatment for internal diseases |
| ZHAO Qi,ZHANG Qiong,XU Qian |
| (The Affiliated Hospital of Hunan Academy of Chinese Medicine，Changsha 410006，Hunan，China;Xiangtan Medicine & Health Vocational College，Xiangtan 411104，Hunan，China) |
| Abstract: |
| Objective：To establish the quality standard for cataplasma for bone and muscle pain.Methods：Thin-layer chromatography (TLC) was performed for Angelica pubescens，Rheum officinale，hematoxylon，and borneol in the prescription，and high-performance liquid chromatography (HPLC) was used to determine the content of emodin in the finished product.Results：The TLC identification method was established for the medicinal materials，including Angelica pubescens，in the cataplasma.With the content of emodin as an index，HPLC was performed on a Kromasil C18 column (250 mm × 4.6 mm，5 μm) with a mobile phase of methanol-0.1% phosphoric acid solution (85∶15)，and at a detection wavelength of 254 nm，emodin was separated effectively from the finished product.The results of methodology showed that this method was feasible.Conclusion：This method is convenient and accurate，with high sensitivity and good repeatability，and therefore，it can provide objective and accurate evidence for developing the quality standard for cataplasma for bone and muscle pain. |
| Key words: external treatment for internal diseases cataplasma for bone and muscle pain quality standard thin-layer chromatography |
