| 摘要: |
| 目的：探讨透骨血竭散外敷对大鼠类风湿性关节炎(RA)的治疗作用及机制。方法：将SPF级雄性SD大鼠40只随机分成正常组、模型组、阳性对照组、透骨血竭散组，每组各10只。除正常组外，其余各组大鼠均予Ⅱ型胶原诱导性关节炎造模法建立RA模型。确定造模成功后第2天开始进行药物干预，阳性对照组予扶他林软膏外敷，透骨血竭散组予自制透骨血竭散外敷，连续14d，正常组、模型组不予处理。观察各组大鼠关节红肿情况；腹主动脉取血，采用ELISA法检测血清中肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）的表达；滑膜免疫组化分析重组人血管内皮生长因子（VEGF）、血清载脂蛋白-1（ApoA-1）的阳性表达。结果：各造模组大鼠每周模型关节炎指数（AI）评分均≥6分，均有明显的RA临床症状和病理学改变；模型组IL-1β、TNF-α的表达量均高于正常组，差异有统计学意义（P＜0.05）。与模型组比较，阳性对照组、透骨血竭散组大鼠IL-1β、TNF-α表达量降低，透骨血竭散组低于阳性对照组，差异均有统计学意义（P＜0.05）；各组大鼠滑膜APOA-1m RNA表达量差异无统计学意义（P＞0.05）；阳性对照组、透骨血竭散组VEGF mRNA表达量均明显低于模型组（P＜0.05），透骨血竭散组低于阳性对照组，差异均有统计学意义（P＜0.05）。结论：透骨血竭散外敷可通过抑制IL-1β、TNF-α及VEGF mRNA的表达量发挥治疗RA的作用，其对滑膜APOA-1的表达无明显影响。 |
| 关键词: 类风湿性关节炎 透骨血竭散 ApoA-1 VEGF TNF-α IL-1β 实验研究 |
| DOI： |
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| Effect of external application of Tougu Xuejie powder on the expression of vascular endothelial growth factor and apolipoprotein A-1 in the synovium of rats with rheumatoid arthritis |
| XIAO Zhi,DENG Wenwen,ZHOU Lingyan |
| (Changsha Third Hospital，Changsha 410015，Hunan，China) |
| Abstract: |
| Objective：To investigate the role and mechanism of external application of Tougu Xuejie powder in the treatment of rats with rheumatoid arthritis (RA).Methods：A total of 40 specific pathogen-free male Sprague-Dawley rats were randomly divided into normal group，model group，positive control group，and Tougu Xuejie powder group，with 10 rats in each group.All rats except those in the normal group were treated with type II collagen to induce arthritis and establish a model of RA.Drug intervention was started on day 2 after successful modeling.The rats in the positive control group were given external application of Voltaren ointment，and those in the Tougu Xuejie powder group were given external application of self-made Tougu Xuejie powder，both for 14 consecutive days；no treatment was given to the rats in the normal group and the model group.Joint swelling was evaluated for all groups；blood samples were collected from the abdominal aorta，and ELISA was used to measure the serum levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)；synovial immunohistochemistry was used to measure the expression of vascular endothelial growth factor (VEGF) and apolipoprotein A-1 (ApoA-1).Results：Each modeling group had an arthritis index of ≥6 and marked clinical symptoms and pathological 〖JP+1〗changes of RA，and the model group had significantly higher expression of IL-1β and TNF-α than the normal group (P＜0.05).Compared with the model group，the positive control group and the Tougu Xuejie powder group had significant reductions in the expression of IL-1β and TNF-α，and the Tougu Xuejie powder group had significantly lower expression than the positive control group (P＜0.05).There was no significant difference in the mRNA expression of ApoA-1 in the synovium between groups (P>0.05).The positive control group and the Tougu Xuejie powder group had significantly lower mRNA expression of VEGF than the model group (P＜0.05)，and the Tougu Xuejie powder group had significantly lower expression than the positive control group (P＜0.05).Conclusion：External application of Tougu Xuejie powder exerts a therapeutic effect on RA by inhibiting the mRNA expression of IL-1β，TNF-α，and VEGF，with little influence on the expression of ApoA-1 in the synovium. |
| Key words: rheumatoid arthritis Tougu Xuejie powder apolipoprotein A-1 vascular endothelial growth factor tumor necrosis factor-α interleukin-1β experimental study |
