| 摘要: |
| 目的：探讨黄连素对β-淀粉样蛋白25-35（Aβ25-35）诱导的大鼠星形胶质细胞的细胞因子及氧化应激的影响。方法：培养第3代的大鼠星形胶质细胞，将其分为空白对照组、模型组、多奈哌齐组，以及黄连素低、高剂量组，分别加入相应药物培养24 h后，模型组和各药物组再加入Aβ25-35。检测各组IL-1β、IL-6、TNF-α mRNA的表达及SOD、MDA水平。结果：Aβ25-35浓度为20 μmol/L时星形胶质细胞增殖率最高；与空白组比较，模型组的IL-1β、IL-6、TNF-α蛋白分泌及其mRNA表达增多，伴随着MDA水平上升，SOD水平下降；与模型组比较，各药物组的IL-1β、IL-6、TNF-α蛋白分泌及其mRNA表达减少，伴随着MDA水平下降，SOD水平上升，尤以黄连素高剂量组疗效最好。结论：黄连素可降低Aβ25-35诱导的大鼠星形胶质细胞的炎性细胞因子，并减少氧化应激。 |
| 关键词: 阿尔茨海默病 星形胶质细胞 黄连素 细胞因子 氧化应激 实验研究 |
| DOI： |
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| Effect of berberine on cytokines and oxidative stress induced by amyloid β-protein25-35 in astrocytes |
| TONG Jingjing,LI Jun,ZHOU Yuwen,HE Xian,ZENG Zhanpeng,GUO Shufen |
| (Chencun Hospital Affiliated to Shunde Hospital，Southern Medical University，Foshan 528313，Guangdong，China;Panyu Hospital of Traditional Chinese Medicine，Guangzhou 511400，Guangdong，China) |
| Abstract: |
| Objective：To investigate the effect of berberine on cytokines and oxidative stress induced by amyloid β-protein25-35 (Aβ25-35） in rat astrocytes.Methods：Third-generation rat astrocytes were cultured and divided into blank control group，model group，donepezil group，and low- and high-dose berberine groups.All groups were cultured with corresponding drugs for 24 hours，and then Aβ25-355 was added to the model group and the three drug groups.The mRNA expression of interleukin-1β (IL-1β)，interleukin-6 (IL-6)，and tumor necrosis factor-α (TNF-α) and the levels of superoxide dismutase (SOD) and malondialdehyde (MDA) were measured.Results：The astrocytes had the highest proliferation rate at the Aβ25-35 concentration of 20 μmol/L.Compared with the blank control group，the model group had significant increases in the mRNA and protein expression of IL-1β，IL-6，and TNF-α，with an increase in MDA and a reduction in SOD.Compared with the model group，the three drug groups had reductions in the mRNA and protein expression of IL-1β，IL-6，and TNF-α，with a reduction in MDA and an increase in SOD，and the high-dose berberine group had the best treatment outcome.Conclusion：Berberine can reduce cytokines and oxidative stress induced by Aβ25-35 in rat astrocytes. |
| Key words: Alzheimer’s disease astrocyte berberine cytokine oxidative stress experimental study |
