| 摘要: |
| 目的：探讨心痛泰颗粒对动脉粥样硬化（AS）小鼠血脂及VCAM-1、TNF-α的影响。方法：将10只野生型小鼠予以普通饲料喂养作为空白对照组，将40只ApoE基因敲除（ApoE-/-）小鼠予以高脂饲料喂养造模，模型制备成功后，将其随机分为模型组、心痛泰低剂量组、心痛泰高剂量组和他汀组，每组各10只，分别予以0.9%氯化钠注射液、心痛泰低剂量、心痛泰高剂量及阿托伐他汀钙片进行胃内给药，连续用药8周。检测各组小鼠用药前后的血清总胆固醇（TC）、三酰甘油（TG）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）及血管细胞黏附分子（VCAM）-1、肿瘤坏死因子-α（TNF-α）的水平变化。结果：与模型组、空白对照组比较，心痛泰低、高剂量组及他汀组可明显降低TC、TG、LDL-C浓度，升高HDL-C血清浓度，控制VCAM-1、TNF-α浓度并减少其阳性表达率，差异均有统计学意义（P＜0.05）；其中，心痛泰高剂量组、他汀组疗效优于心痛泰低剂量组，差异有统计学意义（P<0.05），他汀组与心痛泰高剂量组在降脂疗效方面比较，差异无统计学意义（P>0.05），在降低VCAM-1、TNF-α浓度方面，他汀组优于心痛泰高剂量组（P＜0.05）。结论：心痛泰能够调节血脂、控制炎症，调节VCAM-1、TNF-α的阳性表达，从而干预动脉内斑块的形成及发展，起到预防AS的作用。 |
| 关键词: 动脉粥样硬化 心痛泰颗粒 血脂 VCAM-1 TNF-α 实验研究 |
| DOI： |
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| Effect of Xintongtai granules on blood lipids，vascular cell adhesion molecule-1，and tumor necrosis factor-α in mice with atherosclerosis |
| LUO Li,WEI Jiaming,HUANG Haoyang |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China) |
| Abstract: |
| Objective：To investigate the effect of Xintongtai granules on blood lipids，vascular cell adhesion molecule-1 (VCAM-1)，and tumor necrosis factor-α (TNF-α) in mice with atherosclerosis (AS).Methods：Ten wild-type mice were given normal diet and were selected as blank control group.A total of 40 mice with apolipoprotein E gene knockout were given high-fat diet to establish a model of AS，and after the model was successfully established，these mice were randomly divided into model group，low- and high-dose Xintongtai groups，and statin group，with 10 mice in each group.The mice in the model group were given intragastric administration of 0.9% sodium chloride injection，those in the low- and high-dose Xintongtai groups were given intragastric administration of low- and high-dose Xintongtai，and those in the statin group were given intragastric administration of atorvastatin calcium tablets.The course of treatment was 8 weeks for all group.Serum levels of total cholesterol (TC)，triglyceride (TG)，low-density lipoprotein cholesterol (LDL-C)，high-density lipoprotein cholesterol (HDL-C)，VCAM-1，and TNF-α were measured before and after administration.Results：Compared with the model group and the blank control group，the high- and low-dose Xintongtai groups and the statin group had significant reductions in TC，TG，and LDL-C and a significant increase in serum HDL-C，with controlled concentrations and reduced positive rates of VCAM-1 and TNF-α (P＜0.05).The high-dose Xintongtai group and the statin group had a significantly better treatment outcome than the low-dose Xintongtai group (P＜0.05)，while there was no significant difference in lipid-lowering efficacy between the statin group and the high-dose Xintongtai group (P>0.05).Compared with the high-dose Xintongtai group，the statin group had significantly better reductions in the concentrations of VCAM-1 and TNF-α (P＜0.05).Conclusion：Xintongtai exerts an intervention effect on the formation and development of arterial plaques and helps to prevent AS by regulating blood lipids，controlling inflammation，and regulating the expression of VCAM-1 and TNF-α. |
| Key words: atherosclerosis Xintongtai granules blood lipids vascular cell adhesion molecule-1 tumor necrosis factor-α experimental study |
