| 摘要: |
| 目的：提升天麻头风灵胶囊质量标准，有效控制其制剂质量。方法：采用薄层色谱法，以紫丁香苷和哈巴俄苷为对照，定性鉴别槲寄生和玄参；采用HPLC法对阿魏酸进行含量测定，C18柱（Inertsil ODS-2；4.6mm×250mm，5μm）；流动相：乙腈-0.05%磷酸溶液（16∶84）；检测波长：321nm；流速：1ml/min；柱温：30℃。结果：薄层色谱定性鉴别方法灵敏，专属性强；阿魏酸在9.97～997ng范围内线性良好，r=0.9999；加样回收率为97.52%。结论：本方法简便、重复性好，为天麻头风灵胶囊质量标准提升奠定了基础。 |
| 关键词: 天麻头风灵胶囊 HPLC 阿魏酸 紫丁香苷 哈巴俄苷 |
| DOI： |
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| Improvement of the quality standard for Tianmatoufengling capsules |
| LIU Zhengqing,GUO Yuanman,YANG Hua |
| (Hunan Hansen Pharmaceutical Co.，Ltd.，Yiyang 413000，Hunan，China) |
| Abstract: |
| Objective：To improve the quality standard for Tianmatoufengling capsules，and to effectively control the quality of this prescription.Methods：With syringin and harpagoside as controls，thin-layer chromatography was used for qualitative identification of mistletoe and Radix Scrophulariae.High-performance liquid chromatography was used for the content determination of ferulic acid and was performed on an Inertsil ODS-2 C18column (4.6mm×250mm，5μm) with a mobile phase of acetonitrile-0.05% phosphoric acid (16∶84) at a detection wavelength of 321nm，a flow rate of 1ml/min，and a column temperature of 30℃.Results：Thin-layer chromatography was sensitive and highly specific in qualitative identification.Ferulic acid showed a good linear relationship within the range of 9.97～997ng (r=0.9999)，with an average recovery rate of 97.52%.Conclusion：This method is simple and convenient and has good reproducibility，which lays a foundation for improving the quality standard for Tianmatoufengling capsules. |
| Key words: Tianmatoufengling capsule high-performance liquid chromatography ferulic acid syringin harpagoside |
