| 摘要: |
| 目的：建立滋阴温阳活血颗粒中两种指标性成分（腺苷和山奈酚-3-O-β-D-葡萄糖苷）的同时测定方法。方法：采用HPLC法，选用phenomex Kinetex C18色谱柱（4.6mm×250mm，5μm），0.1%磷酸-乙腈为流动相，梯度洗脱，流速1.0ml/min，柱温30℃，进样量10μm，检测波长254nm；以腺苷和山奈酚-3-O-β-D-葡萄糖苷为指标性成分，共测定10批滋阴温阳活血颗粒。结果：10批颗粒中，腺苷的含量在2.706～4.132mg/g范围内，山奈酚-3-O-β-D-葡萄糖苷的含量在3.692～4.948mg/g范围内。结论：本研究所建立方法快速、准确、高效，能够对滋阴温阳活血颗粒的质量进行科学评价。 |
| 关键词: 滋阴温阳活血颗粒 腺苷 山奈酚-3-O-β-D-葡萄糖苷 含量测定 |
| DOI： |
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| Simultaneous determination of two index components in Ziyin Wenyang Huoxue granules by high-performance liquid chromatography |
| WEN Xiaoyan,TAN Meiying,RONG Liying |
| (Zhaoqing Second People’s Hospital，Zhaoqing 526060，Guangdong，China;Guangdong Second Provincial Traditional Chinese Medicine Hospital & Guangdong Research Institute of Traditional Chinese Medicine Manufacturing Technology，Guangzhou 510095，Guangdong，China) |
| Abstract: |
| Objective：To investigate the method for simultaneous determination of two index components (adenosine and kaempferol-3-O-β-D-glucoside) in Ziyin Wenyang Huoxue granules.Methods：High-performance liquid chromatography was performed on a phenomex Kinetex C18 column (4.6mm×250mm，5μm) with a mobile phase of 0.1% phosphoric acid-acetonitrile for gradient elution，at a flow rate of 1.0mL/min，a column temperature of 30℃，a sample size of 10μm，and a detection wavelength of 254nm.A total of 10 batches of Ziyin Wenyang Huoxue granules were measured with adenosine and kaempferol-3-O-β-D-glucoside as the index components.Results：In the 10 batches of Ziyin Wenyang Huoxue granules，the content of adenosine ranged from 2.706 to 4.132mg/g，and the content of kaempferol-3-O-β-D-glucoside ranged from 3.692 to 4.948mg/g.Conclusion：The method established in this study is quick，accurate，and efficient and can be used for scientific evaluation of the quality of Ziyin Wenyang Huoxue granule. |
| Key words: Ziyin Wenyang Huoxue granule adenosine kaempferol-3-O-β-D-glucoside content determination |
