| 摘要: |
| 目的：观察月华丸（胶囊）对结核分枝杆菌感染巨噬细胞自噬的影响，探寻其抗结核分枝杆菌的药效药理作用及其机制。方法：以月华丸（胶囊）含药血清干预感染结核分枝杆菌小鼠的单核巨噬细胞（RAW264.7），实验共分为5组：阴性对照组、10%月华丸（胶囊）含药血清组、自噬抑制剂3-甲基腺嘌呤（3-MA）+月华丸（胶囊）含药血清组、自噬诱导剂雷帕霉素（Rep）组，另设未被感染的细胞为正常组。采用免疫荧光染色法检测LC3。结果：阴性对照组细胞胞浆中未见LC3蛋白颗粒。与阴性对照组比较，3-MA+月华丸（胶囊）含药血清组细胞胞浆中可见少量LC3蛋白颗粒，10%月华丸（胶囊）含药血清组及Rep组细胞胞浆LC3蛋白颗粒均明显增多，3-MA+月华丸（胶囊）含药血清组多于10%月华丸（胶囊）含药血清组。结论：月华丸（胶囊）含药血清能诱导被耐多药结核菌感染巨噬细胞自噬。 |
| 关键词: 月华丸（胶囊） 耐多药结核菌 LC3 免疫荧光染色法 实验研究 |
| DOI： |
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| Effect of Yuehua pills（capsules）on the autophagy-related protein microtubule-associated protein 1 light chain 3 in multidrug-resistant Mycobacterium tuberculosis infection |
| LIU tingting,OUYANG Jianjun,WU Canrong |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China) |
| Abstract: |
| Objective：To investigate the effect of Yuehua pills（capsules）on the autophagy of macrophages in Mycobacterium tuberculosis infection，as well as the pharmacological action of Yuehua pills（capsules）against Mycobacterium tuberculosis and related mechanism.Methods：RAW264.7 mononuclear macrophages from mice with Mycobacterium tuberculosis infection were treated with serum containing Yuehua pills（capsules）.The experiment was divided into negative control group，serum containing 10% Yuehua pills（capsules）group，autophagy inhibitor 3-methyladenine（3-MA）+serum containing Yuehua pills（capsules）group，and autophagy inducer rapamycin group，and the macrophages without infection were selected as normal group.Immunofluorescent staining was used to measure the level of microtubule-associated protein 1 light chain 3（LC3）.Results：LC3 protein particles were not observed in the cytoplasm in the negative control group.Compared with the negative control group，the 3-MA+serum containing Yuehua pills（capsules）group had a small number of LC3 protein particles in the cytoplasm，and the serum containing 10% Yuehua pills（capsules）group and the rapamycin group had a significant increase in LC3 protein particles in the cytoplasm；the 3-MA+serum containing Yuehua pills（capsules）group had a higher number of LC3 protein particles than the serum containing 10% Yuehua pills（capsules）group.Conclusion：Yuehua pills（capsules）can induce the autophagy of macrophages with Mycobacterium tuberculosis infection. |
| Key words: Yuehua pill（capsule） multidrug-resistant Mycobacterium tuberculosis microtubule-associated protein 1 light chain 3 immunofluorescent staining experimental study |
