| 摘要: |
| 目的：观察健脾消癌方对Wnt信号分子β-连环蛋白（β-catenin）、Axin及靶基因基质金属蛋白酶-7（MMP-7）、CD44的影响，探讨其抗结直肠癌复发转移的作用机制。方法：将对数生长期的HCT116细胞分为空白组，0.9%氯化钠注射液组，5-Fu组及健脾消癌方低、中、高剂量组，分别干预48h后，收集细胞，PCR检测β-catenin、Axin及MMP-7、CD44表达。结果：健脾消癌方含药血清可以降低人结肠癌HCT116细胞中β-catenin mRNA表达水平，上调Axin mRNA表达水平，明显抑制MMP-7 mRNA及CD44 mRNA的表达。结论：健脾消癌方抗结直肠癌转移侵袭的药理作用可能与其下调Wnt/β-catenin信号通路的信号分子β-catenin及上调Axin的表达水平并有效降低靶基因MMP-7、CD44的表达水平有关，这可能是健脾消癌方抗结直肠癌复发转移的重要分子机制之一。 |
| 关键词: 健脾消癌方 结直肠癌 Wnt/β-catenin通路 信号分子 靶基因 实验研究 |
| DOI： |
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| Effect of Jianpi Xiaoai prescription on the Wnt signaling molecules β-catenin and Axin and the target genes matrix metalloproteinase-7 and CD44 in human colon cancer cells |
| TANG Wei，,SONG Cheng，,JIANG Yilan |
| (The Affiliated Hospital of Hunan Academy of Chinese Medicine，Changsha 410006，Hunan，China;Hunan Provincial Cancer Hospital & The Affiliated Tumor Hospital，Xiangya School of Medicine，Central South University，Changsha 410013，Hunan，China) |
| Abstract: |
| Objective：To investigate the effect of Jianpi Xiaoai prescription on the Wnt signaling molecules β-catenin and Axin and target genes matrix metalloproteinase-7 (MMP-7) and CD44 in human colon cancer cells and its mechanism of action in preventing the recurrence and metastasis of colorectal cancer.Methods：HCT116 cells in the logarithmic growth phase were divided into blank group，0.9% sodium chloride injection group，5-fluorouracil (5-Fu) group，and low-，middle-，and high-dose Jianpi Xiaoai prescription groups.The cells were collected after 48 hours of intervention，and PCR was used to measure the expression of β-catenin，Axin，MMP-7，and CD44.Results：In the human colon cancer cell line HCT116，the serum containing Jianpi Xiaoai prescription reduced the mRNA expression of β-catenin，upregulated the mRNA expression of Axin，and significantly inhibited the mRNA expression of MMP-7 and CD44.Conclusion：Jianpi Xiaoai prescription can downregulate β-catenin and upregulate Axin in the Wnt/β-catenin signaling pathway and reduce the expression of the target genes MMP-7 and CD44，which might be one of the important molecular mechanisms of action of Jianpi Xiaoai prescription in preventing the recurrence and metastasis of colorectal cancer. |
| Key words: Jianpi Xiaoai prescription colorectal cancer Wnt/β-catenin signaling pathway signaling molecule target gene experimental study |
