| 摘要: |
| 目的：优选酶解法辅助提取布渣叶总黄酮的工艺条件。方法：以总黄酮提取率为指标，采用单因素试验、正交试验法，分别考察酶种类、酶用量、pH值、酶解时间及乙醇浓度对提取工艺的影响。结果：最佳工艺条件为复合酶（纤维素酶∶果胶酶=1∶1）用量1%，乙醇浓度60%，酶解1h，总黄酮提取率为20.90%，较未经酶解法提高了4.85%。结论：优选的工艺简单高效，为布渣叶总黄酮的开发利用提供了实验依据。 |
| 关键词: 布渣叶 总黄酮 复合酶 实验研究 |
| DOI： |
|
|
| Optimization of the process of enzymolysis in assisting the extraction of total flavonoids from Microcos paniculata |
| CHEN Xue-ting，,XU Wen-jie，,LI Zhi-yong |
| (Guangdong Second Provincial Traditional Chinese Medicine Hospital/Guangdong Research Institute of Traditional Chinese Medicine Manufacturing Technology，Guangzhou 510095，Guangdong，China) |
| Abstract: |
| Objective：To optimize the process conditions for enzymolysis in assisting the extraction of total flavonoids from Microcos paniculata.Methods：With the extraction rate of total flavonoids as an index，the single-factor experiment or orthogonal test was used to assess the influence of type of enzyme，amount of enzyme used，pH value，time of enzymolysis，and alcohol concentration on extraction process.Results：The optimal process conditions were compound enzyme（cellulose:pectinase=1∶1）with an amount of 1%，an alcohol concentration of 60%，and a duration of enzymolysis of 1 hour.The extraction rate of total flavonoids was 20.90%，which was 4.85% higher than the extraction rate obtained by unenzymatic hydrolysis.Conclusion：The optimized process is simple and efficient and provides an experimental basis for the exploitation and utilization of total flavonoids from Microcos paniculata. |
| Key words: Microcos paniculata total flavonoids compound enzyme experimental study |
