| 摘要: |
| 目的：建立四甲丸中2种活性成分哈巴俄苷、肉桂酸的含量测定方法。方法：色谱条件为C18色谱柱（4.6mm×250mm，5μm）；流动相为乙腈-1%醋酸水溶液，体积比为25∶75，等度洗脱；流速：1mL/min，柱温：25℃；检测波长：278nm。结果：在该色谱条件下，哈巴俄苷、肉桂酸分离良好，质量浓度分别在5.36～134.06μg/mL（r=0.9999）、5.28～131.95μg/mL（r=0.9997）时，与其峰面积线性关系良好。加样回收率分别为103.6%（1.20%）、100.4%（1.30%）（n=6）。结论：本试验所建立的方法简便、准确、重复性好，可用于四甲丸的质量控制。 |
| 关键词: 四甲丸 哈巴俄苷 肉桂酸 含量测定 |
| DOI： |
|
|
| A quantitative method for the quality control of Sijia pills |
| DENG Man-jing，,GUO Hong-ling，,XIAO Ke-yue |
| (Changsha Hospital of Traditional Chinese Medicine/Changsha Eighth Hospital，Changsha 410001，Hunan，China) |
| Abstract: |
| Objective：To investigate the method for content determination of two active components，harpagoside and cinnamic acid，in Sijia pills.Methods：High-performance liquid chromatography was performed on a C18 column（4.6mm×250mm，5μm）with a mobile phase of acetonitrile-1% acetic acid solution（V/V 25∶75）for isocratic elution，at a flow rate of 1mL/min，a column temperature of 25℃，and a detection wavelength of 278nm.Results：Under such chromatographic conditions，harpagoside and cinnamic acid were well isolated，with a good linear relationship with peak area at a mass concentration of 5.36～134.06μg/mL（r=0.9999）and 5.28～131.95μg/mL（r=0.9997)，respectively.The average recovery rate was 103.6%（1.20%）and 100.4%（1.30%） ，respectively（n=6).Conclusion：The method established in this study is simple，convenient，and accurate，with good repeatability，and can be used for the quality control of Sijia pills. |
| Key words: Sijia pills harpagoside cinnamic acid content determination |
