| 摘要: |
| 目的：测定湖南洪江国家黄精标准化基地多花黄精中的多糖含量，为多花黄精的进一步开发利用及药材质量标准研究奠定基础。方法：以葡萄糖为标准品，采用硫酸-蒽酮试剂显色，应用紫外-可见分光光度法测定多花黄精多糖含量。结果：葡萄糖在3.28～19.68μg/mL范围内线性良好（r=0.9992），多花黄精多糖平均含量为8.05%。结论：洪江种植基地的多花黄精品质优良，洪江是道地黄精药材种植理想基地。 |
| 关键词: 多花黄精 黄精多糖 含量测定 |
| DOI： |
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| Content determination of polysaccharides in Polygonatum cyrtonema Hua by ultraviolet-visible spectrophotometry |
| ZENG Ting，,ZHOU Fang，,TANG Yan-ran |
| (Laboratory of Traditional Chinese Medicine & Folk Medicine Innovation and International Development，Hunan University of Chinese Medicine，Changsha 410208，Hunan，China) |
| Abstract: |
| Objective：To investigate the method for content determination of polysaccharides in Polygonatum cyrtonema Hua from Hunan Hongjiang National Polygonatum sibiricum Standardized Base，and to lay a foundation for further development and utilization of Polygonatum cyrtonema Hua and research on quality standards of medicinal materials.Methods：With glucose as the standard substance，the sulfuric acid-anthrone method and ultraviolet-visible spectrophotometry were used to determine the content of polysaccharides in Polygonatum cyrtonema Hua.Results：Glucose showed a good linear relationship within the range of 3.28～19.68μg/mL（r=0.9992），and the average content of polysaccharides in Polygonatum cyrtonema Hua was 8.05%.Conclusion：Polygonatum cyrtonema Hua from the Hongjiang plantation base has good quality，and Hongjiang is an ideal base for the plantation of genuine Polygonatum sibiricum medicinal material. |
| Key words: Polygonatum cyrtonema Hua Polygonatum sibiricum polysaccharides content determination |
