| 摘要: |
| 目的：观察槲皮素对人成骨肉瘤MG-63细胞增殖和凋亡的影响。方法：不同浓度的槲皮素作用于体外培养的MG-63细胞，四甲基偶氮唑盐（MTT）法检测槲皮素对MG-63细胞增殖的抑制能力，流式细胞术（FCM）检测槲皮素对MG-63细胞凋亡的影响。结果：槲皮素可显著抑制MG-63细胞增殖，具有明显剂量和时间依赖性；槲皮素可明显诱导MG-63细胞凋亡。结论：槲皮素能够抑制MG-63细胞增殖，诱导MG-63细胞凋亡。 |
| 关键词: 槲皮素 人成骨肉瘤MG-63细胞 细胞增殖 细胞凋亡 实验研究 |
| DOI： |
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| Effect of quercetin on in vitro proliferation and apoptosis of human osteosarcoma MG-63 cells |
| SUN Nan-yang，,FU Fang-zi，,TAN Jiang-bo |
| (The Second Affiliated Hospital of Hunan University of Chinese Medicine，Changsha 410005，Hunan，China;Haikou Hospital Affiliated to Xiangya School of Medicine，Central South University，Haikou 570208，Hainan，China;Xiangdong Hospital Affiliated to Hunan Normal University，Zhuzhou 412200，China) |
| Abstract: |
| Objective：To investigate the effect of quercetin on in vitro proliferation and apoptosis of human osteosarcoma MG-63 cells.Methods：Different concentrations of quercetin were used for in vitro culture of MG-63 cells.MTT assay was used to assess the inhibitory effect of quercetin on the proliferation of MG-63 cells，and flow cytometry was used to evaluate the effect of quercetin on the apoptosis of MG-63 cells.Results：Quercetin significantly inhibited the proliferation of MG-63 cells in a dose- and time-dependent manner.It also induced the poptosis of MG-63 cells.Conclusion：Quercetin can inhibit the proliferation of MG-63 cells and induce the apoptosis of MG-63 cells. |
| Key words: quercetin human osteosarcoma MG-63 cell cell proliferation cell apoptosis experimental study |
