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基于网络药理学探讨阳和汤治疗三阴性乳腺癌的作用机制
程阳子,任翠翠,于桂萍
0
(西安市第九医院,陕西 西安,710054;西安市第一医院,陕西 西安,710002)
摘要:
目的:运用网络药理学方法探讨阳和汤治疗三阴性乳腺癌的潜在作用机制。方法:通过TCMSP数据库和HERB数据库筛选得到阳和汤的有效成分并预测其相关作用靶点,构建有效成分-靶点相互作用网络图,通过DisGeNET和GeneCards数据库获取三阴性乳腺癌的相关靶点,运用仙桃学术工具在线venny分析获取阳和汤与三阴性乳腺癌的交集靶点,STRING数据库及Cytoscape软件构建蛋白质-蛋白质相互作用(PPI)网络图,并进行基因本体(GO)功能及京都基因与基因组百科全书(KEGG)通路富集分析。进一步通过FerrDb数据库获取铁死亡相关靶点,并与阳和汤、三阴性乳腺癌靶点取交集,进行PPI网络图构建及GO功能、KEGG通路富集分析。结果:筛选得到阳和汤的有效成分86个,其中核心成分为槲皮素、山柰酚等,对应作用靶点207个,三阴性乳腺癌的相关靶点1748个,两者共同靶点89个,其中核心靶点为蛋白激酶B(Akt1)、丝裂原活化蛋白激酶1(MAPK1)、磷脂酰肌醇-3激酶调节亚基1(PIK3R1)等,通过KEGG分析发现,阳和汤治疗三阴性乳腺癌涉及PI3K-Akt信号通路、黏附连接等主要信号通路。进一步获取铁死亡的调控靶点487个,其中MAPK1、过氧化物酶体增殖物激活受体γ(PPARγ)为铁死亡参与阳和汤治疗三阴性乳腺癌的核心靶点。结论:阳和汤治疗三阴性乳腺癌主要涉及多个关键靶点和信号通路,推测铁死亡通路可能是其重要的作用机制。
关键词:  三阴性乳腺癌  阳和汤  铁死亡  网络药理学  脂代谢通路
DOI:
Mechanism of action of Yanghe decoction in treatment of triple-negative breast cancer: A study based on network pharmacology
CHENG Yangzi,REN Cuicui,YU Guiping
(Xi’an No.9 Hospital,Xi’an 710054,Shaanxi,China;Xi’an No.1 Hospital,Xi’an 710002,Shaanxi,China)
Abstract:
Objective: To investigate the potential mechanism of action of Yanghe decoction in the treatment of triple-negative breast cancer based on network pharmacology.Methods: TCMSP and HERB databases were used to obtain the effective constituents of Yanghe decoction and predict their action targets,and an effective constituent-target interaction network was constructed.DisGeNET and GeneCards databases were used to obtain the targets associated with triple-negative breast cancer,and Xiantao Venny tool was used to obtain the intersecting targets of Yanghe decoction and triple-negative breast cancer.STRING database and Cytoscape software were used to construct a protein-protein interaction (PPI) network,and gene ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed.FerrDb database was used to obtain the targets associated with ferroptosis,which were intersected with the targets of Yanghe decoction and triple-negative breast cancer to construct a PPI network and perform GO functional enrichment analysis and KEGG pathway enrichment analysis.Results: A total of 86 effective constituents were obtained for Yanghe decoction,among which core components included quercetin and kaempferol,and there were 207 corresponding action targets.A total of 1748 targets were obtained for triple-negative breast cancer,and there were 89 common targets between the two groups of targets,with the core targets including protein kinase B,mitogen-activated protein kinase 1 (MAPK1),and phosphatidylinositol 3-kinase regulatory subunit 1.KEGG analysis showed that the PI3K-Akt signaling pathway and adherens junction were the main signaling pathways involved in the treatment of triple-negative breast cancer by Yanghe decoction.A total of 487 regulatory targets were obtained for ferroptosis,among which MAPK1 and peroxisome proliferator-activated receptor gamma were the core targets of ferroptosis involved in the treatment of triple-negative breast cancer by Yanghe decoction.Conclusion: The treatment of triple-negative breast cancer by Yanghe decoction involves multiple key targets and signaling pathways,and the ferroptosis pathway might be an important mechanism of action.
Key words:  triple-negative breast cancer  Yanghe decoction  ferroptosis  network pharmacology  lipid metabolic pathway

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