| 摘要: |
| 目的：采用实验研究的方法评价自拟中药方剂治疗阴道炎的药效和毒性。方法：将40只豚鼠随机分为模型对照组，地塞米松阳性药物组（0.25 mg/ml），中药方剂高（52.00 mg/ml）、中（13.00 mg/ml）、低（3.25 mg/ml）剂量组，每组各8只。于各组豚鼠右后脚背剃毛处分别涂抹相应药物，末次给药15 min后，在点刺处依次涂抹不同浓度磷酸组胺直至豚鼠回头舔舐。大鼠脚趾肿胀模型实验分组同上。将3%甲醛注入各组大鼠脚趾内建立肿胀模型，造模成功后均匀涂抹相应药物于炎症患处。将新西兰大白兔分为染毒组和对照组，每组各3只，分别注射52 mg/ml中药原液、0.9%氯化钠注射液，每24 h重复1次，连续5 d。统计单位时间豚鼠搔抓次数及耐受磷酸组胺总量；观察大鼠脚趾肿胀消退情况并记录脚趾厚度变化；观察实验兔阴道是否水肿，HE染色检测阴道黏膜有无明显变化。结果：与模型对照组相比，中药方剂高、中、低剂量组豚鼠单位时间搔抓次数均减少，耐受磷酸组胺总量均增加，但中药方剂低剂量组效果更明显，且显著优于地塞米松阳性药物组（P<0.05）；中药方剂高、中剂量组消肿速率快，与地塞米松阳性药物组比较，差异无统计学意义（P＞0.05）；给药组阴道组织无明显充血和红肿现象，阴道黏膜层次清晰，与模型对照组切片无明显差异；染毒组实验兔阴道黏膜刺激指数为0.2。结论：该自制中药方剂对阴道炎具有抗炎、消肿、止痒的功效，且对阴道皮肤黏膜无刺激性，无明显毒副作用。 |
| 关键词: 阴道炎 中药方剂 药效 毒性评价 |
| DOI： |
|
|
| Pharmacodynamics and toxicity of a traditional Chinese medicine prescription in treatment of vaginitis：An experimental study |
| HUI Dandan,CHEN Yimeng,LI Yunxiang |
| (School of Biological Science and Engineering，Shaanxi University of Technology，Hanzhong 723000，Shaanxi，China) |
| Abstract: |
| Objective：To investigate the pharmacodynamics and toxicity of a self-made traditional Chinese medicine (TCM) prescription in the treatment of vaginitis based on an experimental study.Methods：A total of 40 guinea pigs were randomly divided into model control group，dexamethasone positive drug group (0.25 mg/ml)，high-dose TCM prescription group (52.00 mg/ml)，middle-dose TCM prescription group (13.00 mg/ml)，and low-dose TCM prescription group (3.25 mg/ml)，with 8 guinea pigs in each group.The corresponding drug was rubbed at the instep of the right rear foot after shaving，and at 15 minutes after the last administration，different concentrations of histamine phosphate were rubbed at the pricking site in proper order until the guinea pig turned back and licked.In the experiment with the rat model of toe swelling，the rats were divided into the same groups.The toe of the rat was injected with 3% formaldehyde to establish a model of toe swelling，and after successful modeling，the corresponding drug was rubbed at the site of the inflammation.New Zealand white rabbits were divided into exposure group and control group，with 3 rabbits in each group，and the rabbits in the exposure group were injected with 52 mg/ml TCM stock solution，while those in the control group were injected with 0.9% sodium chloride injection，once every 24 hours for 5 consecutive days.A statistical analysis was performed for the number of scratches per unit time and the total amount of histamine phosphate tolerated by the guinea pigs；regression of toe swelling was observed，and the change in toe thickness was recorded；vaginal edema of the experimental rabbits was observed，and HE staining was used to observe the change in vaginal mucosa.Results：Compared with the model control group，the high-，middle-，and low-dose TCM prescription groups had a significant reduction in the number of scratches per unit time and a significant increase in the total amount of histamine phosphate tolerated by the guinea pigs，and the low-dose TCM prescription group tended to have better changes，showing a significantly better effect than the dexamethasone positive drug group (P<0.05).The high- and middle-dose TCM prescription groups had rapid subsidence of swelling，with no significant difference compared with the dexamethasone positive drug group (P>0.05).The administration group had no marked congestion or swelling of vaginal tissue and had clear layers of vaginal mucosa，with no significant differences compared with the sections of the model control group.The stimulation index of vaginal mucosa was 0.2 in the exposure group.Conclusion：The self-made TCM prescription has anti-inflammatory，detumescence，and itching-relieving effects against vaginitis，without stimulation on vaginal mucosa or obvious toxic and side effects. |
| Key words: vaginitis traditional Chinese medicine prescription pharmacodynamics toxicity evaluation |
