| 摘要: |
| 目的：基于网络药理学方法探讨玉屏风散治疗过敏性紫癜（HSP)的作用机制。方法：利用TCMSP、Pubchem、Swiss target prediction等数据库平台，对玉屏风散的有效活性成分和有效靶点基因进行检索、收集和筛选。通过Genecards、Disgenet、OMIM数据库查询和挖掘HSP的相关基因和玉屏风散治疗HSP的交集靶点，STRING数据库和Cytoscape 3.9.1 软件构建活性-成分-靶点可视化网络和可视化分析。运用DAVID数据库进行基因本体（GO）功能及京都基因与基因组百科全书（KEGG）通路富集分析。结果：筛选出45个玉屏风散活性成分，212个药物靶点，294个疾病靶点和35个交集靶点，对关键靶点进行蛋白质-蛋白质互作（PPI）网络构建，涉及的主要基因有肿瘤坏死因子（TNF）、白细胞介素-6、血管内皮生长因子A、白细胞介素-1β、肿瘤蛋白p53、基质金属蛋白酶-9、趋化因子c-c配体2、白细胞介素-10、丝氨酸蛋白酶抑制因子肽酶抑制因子、CXC趋化因子配体8等。GO功能富集分析得到条目数337个，其中生物过程条目284个，分子功能条目35个，细胞组成条目22个。KEGG通路富集分析得到信号通路101条，主要涉及高级糖基化终末产物-受体信号通路、流体剪切应力和动脉粥样硬化信号通路、脂质和动脉硬化信号通路、疟疾信号通路、白细胞介素-17信号传导通路、TNF信号通路、核因子-κB信号通路等。结论：玉屏风散可能通过多成分、多靶点、多通路治疗HSP。 |
| 关键词: 玉屏风散 过敏性紫癜 作用机制 网络药理学 |
| DOI： |
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| Mechanism of action of Yupingfeng powder in treatment of Henoch-Schonlein purpura：A study based on network pharmacology |
| LIU Yi,AN Zhenxiang,HE Xiuyi |
| (Bijie Medical College，Bijie 551700，Guizhou，China;Guizhou University of Traditional Chinese Medicine，Guiyang 550025，Guizhou，China) |
| Abstract: |
| Objective:To investigate the mechanism of action of Yupingfeng powder in the treatment of Henoch-Schonlein purpura (HSP) based on network pharmacology.Methods：Databases including TCMSP，Pubchem，and Swiss target prediction were used to obtain the active components of Yupingfeng powder and effective target genes.Databases such as Genecards，Disgenet，and OMIM were used to obtain the intersecting targets between the genes associated with HSP and the targets of Yupingfeng powder in the treatment of HSP.STRING database and Cytoscape 3.9.1 software were used to construct an active component-target visualized network and perform a visual analysis.DAVID database was used to perform gene ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis.Results：The above analyses obtained 45 active components of Yupingfeng powder，212 drug targets，294 disease targets，and 35 intersecting targets.A protein-protein interaction network was established for the key genes，and the main genes involved included tumor necrosis factor，interleukin-6，vascular endothelial growth factor A，interleukin-1β，tumor protein p53，matrix metalloproteinase-9，chemokine (C-C motif) ligand 2，interleukin-10，serpin peptidase inhibitor，and CXC chemokine ligand 8.GO functional enrichment analysis obtained 337 GO terms，among which there were 284 biological processes，35 molecular functions，and 22 cellular components.KEGG pathway enrichment analysis obtained 101 signaling pathways，including the AGE-RAGE signaling pathway，the fluid shear stress and atherosclerosis signaling pathway，the lipid and atherosclerosis signaling pathway，the malaria signaling pathway，the interleukin-17 signaling pathway，the tumor necrosis factor signaling pathway，and the nuclear factor-kappa B signaling pathway.Conclusion：Yupingfeng powder exerts a therapeutic effect on HSP via multiple components，targets，and pathways. |
| Key words: Yupingfeng powder Henoch-Schonlein purpura mechanism of action network pharmacology |
