| 摘要: |
| 目的：探讨茯砖茶对高脂血症APOE-/-（载脂蛋白E敲除）小鼠瘦素抵抗（LR）的影响。方法：将32只8周龄APOE-/-小鼠随机分为模型组（灌胃等体积蒸馏水），茯砖茶低（1.44 g/kg·d）、高（2.16 g/kg·d）剂量组，阿托伐他汀组（2.88 mg/kg·d），每组各 8 只，高脂饮食喂养并同时予以相应药物连续灌胃干预4个月。另选8只8周龄C57BL/6小鼠作为正常组，予以普通饲料喂养。比较各组小鼠体质量、腹部脂肪质量、血清血脂［三酰甘油（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）］、瘦素（LEP）、胰岛素（INS）、脂联素（ADP）；HE染色比较各组脂肪组织细胞差异，免疫组化比较各组脑组织瘦素受体（LEPR）表达；RT-qPCR检测各组小鼠脂肪组织中LEP、肿瘤坏死因子-α（TNF-α)、白细胞介素-6（IL-6）的mRNA表达。结果：与模型组比较，茯砖茶高、低剂量组体质量，腹部脂肪质量，血清TG、TC、LDL-C、LEP、INS水平及脂肪组织中的LEP、TNF-α、IL-6的mRNA表达均下降，脂肪细胞体积均减小，细胞排列较为有序（P＜0.01或P＜0.05），HDL-C、ADP水平及下丘脑中LEPR表达均升高（P＜0.05）。结论：茯砖茶可以通过改善LR，对抗高脂血症，其作用机制可能与其能调控脂肪组织LEP、ADP的释放及血清INS含量，增强下丘脑组织中LEPR的表达有关。 |
| 关键词: 高脂血症 茯砖茶 APOE-/- 瘦素抵抗 胰岛素 脂联素 |
| DOI： |
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| Effect of Fuzhuan Tea on leptin resistance in APOE-/- mice with hyperlipidemia |
| ZHANG Wenjiang,LIU Baiyan,CHEN Xuemei |
| (Shaanxi University of Chinese Medicine，Xianyang 712046，Shaanxi，China;Hunan Academy of Chinese Medicine，Changsha 410013，Hunan，China;Hunan University of Chinese Medicine，Changsha 410208，Hunan，China) |
| Abstract: |
| Objective: To investigate the effect of Fuzhuan Tea on leptin resistance (LR) in APOE-/- (apolipoprotein E knockout) mice with hyperlipidemia.Methods: A total of 32 APOE-/- mice，aged 8 weeks，were randomly divided into model group (an equal volume of distilled water by gavage)，low- and high-dose Fuzhuan Tea groups (1.44 and 2.16 g/kg/day，respectively)，and atorvastatin group (2.88 mg/kg/day)，with 8 mice in each group.The mice were given high-fat diet and the corresponding drug by gavage for 4 consecutive months.In addition，8 C57BL/6 mice，aged 8 weeks，were selected as normal group and were given normal diet.The above groups were compared in terms of body weight，abdominal fat weight，serum lipids ［triglyceride (TG)，total cholesterol (TC)，low-density lipoprotein cholesterol (LDL-C)，and high-density lipoprotein cholesterol (HDL-C)］，leptin (LEP)，insulin (INS)，and adiponectin (ADP)；HE staining was used to compare adipose tissue cells between groups，and immunohistochemistry was used to measure the expression of leptin receptor (LEPR) in brain tissue；RT-qPCR was used to measure the mRNA expression levels of LEP，tumor necrosis factor-α (TNF-α)，and interleukin-6 (IL-6) in adipose tissue of mice.Results: Compared with the model group，the low- and high-dose Fuzhuan Tea groups had significant reductions in body weight，abdominal fat weight，the serum levels of TG/TC/LDL-C/LEP/INS，the mRNA expression levels of LEP/TNF-α/IL-6 in adipose tissue，and adipocyte volume，with relatively ordered arrangement of cells (P＜0.01 or P＜0.05)，as well as significant increases in the levels of HDL-C and ADP and the expression level of LEPR in the hypothalamus (P＜0.05).Conclusion: Fuzhuan Tea can exert a therapeutic effect on hyperlipidemia by improving LR，possibly by regulating the release of LEP and ADP in adipose tissue and the content of INS in serum and enhancing the expression of LEPR in the hypothalamus. |
| Key words: hyperlipidemia Fuzhuan Tea APOE-/- leptin resistance insulin adiponectin |
