| 摘要: |
| 目的：运用网络药理学方法分析郭维琴教授经验方益气泻肺汤治疗慢性心力衰竭的潜在作用机制。方法：借助TCMSP、SwissTargetPrediction、DrugBank等数据库，检索益气泻肺汤主要药物的活性成分作用靶点；利用GeneCards、DrugBank、DisGeNET等数据库检索慢性心力衰竭的相关靶点；借助Cytoscape 3.2.1软件构建益气泻肺汤活性成分靶点-慢性心力衰竭疾病靶点网络；通过基因本体（GO）功能和京都基因与基因组百科全书（KEGG）通路富集分析预测益气泻肺汤治疗慢性心力衰竭的潜在分子生物过程和信号通路。结果：筛选得到益气泻肺汤活性成分靶点795个，慢性心力衰竭疾病靶点356个，取交集得到94个关键靶点，构建益气泻肺汤活性成分靶点-慢性心力衰竭疾病靶点网络，得到肿瘤坏死因子（TNF）、肿瘤抑制蛋白（TP53）、内皮素1（EDN19）、沉默调节蛋白1（SIRT1）、血管紧张素原（AGT）、C反应蛋白（CRP）、类胰岛素生长因子（IGF1）、连环蛋白（CTNNB1）8个核心靶点，此为益气泻肺汤治疗慢性心力衰竭的重要潜在靶点；GO 功能和KEGG 通路富集分析显示益气泻肺汤治疗慢性心力衰竭可能的潜在通路主要有钙信号通路、肾素分泌等。结论：本研究初步揭示了益气泻肺汤有效成分通过多靶点调控、参与多种信息通路和生物学过程治疗慢性心力衰竭的作用机制。 |
| 关键词: 慢性心力衰竭 益气泻肺汤 网络药理学 作用机制 |
| DOI： |
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| Mechanism of action of Yiqi Xiefei decoction in treatment of chronic heart failure：A study based on network pharmacology |
| XU Qian,KOU Lanjun,LIANG Jinpu |
| (The First Clinical Medical College of Beijing University of Chinese Medicine，Beijing 100029，China;Dongzhimen Hospital，Beijing University of Chinese Medicine，Beijing 100700，China) |
| Abstract: |
| Objective：To investigate the potential mechanism of action of Yiqi Xiefei decoction used by Professor Guo Weiqin in the treatment of chronic heart failure based on network pharmacology.Methods：Databases such as TCMSP，SwissTargetPrediction，and DrugBank were used to search for the action targets of the active components of the main drugs in Yiqi Xiefei decoction；databases such as GeneCards，DrugBank，and DisGeNET were used to obtain the targets associated with chronic heart failure；Cytoscape 3.2.1 was used to construct a network of the targets of the active components of Yiqi Xiefei decoction and the disease targets of chronic heart failure；gene ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were used to predict the potential molecular biological processes and signaling pathways in the treatment of chronic heart failure by Yiqi Xiefei decoction.Results：The above screening obtained 795 targets for the active components of Yiqi Xiefei decoction and 356 disease targets for chronic heart failure，and these two groups of targets were intersected to obtain 94 key targets.A network was constructed for the targets of the active components of Yiqi Xiefei decoction and the disease targets of chronic heart failure and obtained 8 core targets，i.e.，tumor necrosis factor，TP53，EDN19，silent information regulator 1，angiotensinogen，C-reactive protein，IGF1，and CTNNB1，which were important potential targets for the treatment of chronic heart failure by Yiqi Xiefei decoction.The GO functional enrichment analysis and the KEGG pathway enrichment analysis showed that calcium signaling pathway and renin secretion were potential pathways involved the treatment of chronic heart failure by Yiqi Xiefei decoction.Conclusion：This study preliminarily reveals the mechanism of action of Yiqi Xiefei decoction in the treatment of chronic heart failure by regulating multiple targets and participating in various signaling pathways and biological processes. |
| Key words: chronic heart failure Yiqi Xiefei decoction network pharmacology mechanism of action |
