| 摘要: |
| 目的：优选老鹳草中柯里拉京的提取工艺条件，并通过对 α-淀粉酶活力的抑制作用初步判断柯里拉京的降糖作用。方法：以柯里拉京提取率为指标，采用单因素考察提取方法、提取溶剂和提取次数对老鹳草柯里拉京提取率的影响；在单因素试验的基础上，采用正交设计法优化老鹳草水提取工艺条件。结果：老鹳草水提取的最佳工艺条件为加水回流提取2次，用量8，6倍，提取时间 1.5，1.5 h，在此条件下，老鹳草柯里拉京的提取率 1.2%，影响老鹳草柯里拉京提取率的因素从大到小依次为：加水倍数＞提取时间＞浸泡时间。结论：通过对α-淀粉酶活力的抑制作用测定，表明老鹳草中柯里拉京有较好的降糖作用。 |
| 关键词: 老鹳草 柯里拉京 提取工艺 α-淀粉酶 抑制作用 |
| DOI： |
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| Optimization of the extraction process of Geranium wilfordii maxim.and its inhibitory effect on α-amylase |
| CHEN Dan,LI Wei,LI Xiaolan |
| (Xiangtan Medicine & Health Vocational College，Xiangtan 411102，Hunan，China;Hunan Academy of Chinese Medicine，Changsha 410013，Hunan，China) |
| Abstract: |
| Objective：To optimize the extraction process conditions of corilagin in Geranium wilfordii maxim.，and to investigate the hypoglycemic effect of corilagin based on its inhibitory effect on the activity of α-amylase.Methods：With the extraction rate of corilagin as an index，the univariate analysis was used to investigate the influence of extraction method，extraction solvent，and times of extraction on the extraction rate of corilagin in Geranium wilfordii maxim.，and then an orthogonal design was used to optimize the water extraction process conditions for Geranium wilfordii maxim.Results：The optimal water extraction process conditions for Geranium wilfordii maxim.were reflux extraction with water twice，an amount of 8，6 times，and an extraction time of 1.5，1.5 hours for both times of extraction，and under such conditions，the extraction rate of corilagin Geranium wilfordii maxim.was 1.2%.The folds of water added was the greatest influencing factor for the extraction rate of corilagin，followed by extraction time and soak time.Conclusion：The inhibitory effect of corilagin in Geranium wilfordii maxim.on the activity of α-amylase is measured to show that corilagin has a good hypoglycemic effect. |
| Key words: Geranium wilfordii maxim. corilagin extraction process α-amylase inhibitory effect |
