| 摘要: |
| 目的：采用气相色谱-串联质谱法（GC-MS）分析技术建立定性和定量检测中药材茯苓中25种禁用农药残留的方法。方法：以乙腈为提取溶剂，对干燥茯苓样品进行提取、净化、浓缩等预处理后，经气相色谱-三重四极杆串联质谱分析测定，并用内标法定量。结果：茯苓中25种禁用农药在1～100 ng/ml范围内具有良好的线性关系，相关系数均在0.9900以上；检出限在0.00040～0.0100 mg/kg；定量限在0.000667～0.0333 mg/kg；以上检测均符合农药残留的检测要求。加标回收实验的平均加标率为70.1%～123.3%，加标率和加标样品的重复性验证结果也均符合标准要求。18批茯苓样品均未检出25种农药残留。结论：该方法简便快速、灵敏度高、重现性好，可适用于多种中药材中禁用农药残留的检测。 |
| 关键词: 气相色谱-串联质谱法 禁用农药 中药材 茯苓 |
| DOI： |
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| Determination of forbidden pesticide residue in Chinese medicinal material Poria cocos based on gas chromatography-mass spectroscopy |
| YUAN Nong,DU Juan,LONG Huiling |
| (Hunan Center for Drug Evaluation and ADR Monitoring，Changsha 410013，Hunan，China) |
| Abstract: |
| Objective：To establish a method for qualitative and quantitative determination of the residue of 25 forbidden pesticides in the Chinese medicinal material Poria cocos using gas chromatography-mass spectroscopy.Methods：With acetonitrile as the extraction solvent，dried Poria cocos samples were pretreated by extraction，purification，and concentration，and then gas chromatography-triple quadrupole tandem mass spectrometry was used for analysis and determination，with the internal standard method for quantification.Results：The 25 forbidden pesticides in Poria cocos showed a good linear relationship within the range of 1-100 ng/mL，with a correlation coefficient of >0.9900.The limit of detection had a range of 0.00040-0.0100 mg/kg，and the limit of quantification had a range of 0.000667-0.0333 mg/kg.All the results above met the requirements for pesticide residue.The mean recovery rate was 70.1%-123.3%，and the results of recovery rate and repeatability also met the standard requirements.The residue of 25 pesticides was not detected in 18 batches of Poria cocos samples.Conclusion：This method is simple and rapid with high sensitivity and good repeatability and can be used for determining the residue of various forbidden pesticides in Chinese medicinal materials. |
| Key words: gas chromatography-mass spectroscopy forbidden pesticide Chinese medicinal material Poria cocos |
