| 摘要: |
| 目的：观察温肺降浊方对血管性痴呆（VaD）大鼠Nogo-A和NgR mRNA及其蛋白表达的影响。方法：将30只大鼠随机分为假手术组、模型组、中药组，每组各10只。采用双侧颈总动脉永久性结扎方法制作VaD大鼠模型，造模成功后中药组予温肺降浊方灌胃，假手术组、模型组予以等量0.9%氯化钠注射液灌胃，疗程均为4周。采用Morris水迷宫行为学测试大鼠学习记忆能力；RT-PCR、Western blot检测各组大鼠Nogo-A和NgR mRNA及其蛋白表达水平，透射电镜观察神经元突触结构变化。结果：与假手术组比较，模型组大鼠逃避潜伏期延长、穿越平台次数减少（P<0.01或P<0.05）；海马中Nogo-A和NgR蛋白及mRNA表达增多（P<0.01）。与模型组比较，中药组逃避潜伏期缩短、穿越平台次数增加（P<0.05）；海马中Nogo-A和NgR mRNA及其蛋白减少（P<0.05）。海马神经元突触及细胞器完整、突触间隙及小泡正常。结论：温肺降浊方可改善VaD大鼠学习记忆能力，其机制可能与降低Nogo-A和NgR mRNA及蛋白表达含量，促进神经突触再生，改善海马突触可塑性有关。 |
| 关键词: 血管性痴呆 神经元突触可塑 温肺降浊方 Nogo-A NgR 实验研究 |
| DOI： |
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| Effect of Wenfei Jiangzhuo prescription on neuronal synaptic plasticity in rats with vascular dementia by regulating the expression of Nogo-A and NgR |
| XIANG Junjun,CHEN Wei,LAI Jingjing |
| (The First Affiliated Hospital of Guangxi University of Chinese Medicine，Nanning 530023，Guangxi，China) |
| Abstract: |
| Objective：To investigate the effect of Wenfei Jiangzhuo prescription on the mRNA and protein expression levels of Nogo-A and NgR in rats with vascular dementia (VaD).Methods：A total of 30 rats were randomly divided into sham-operation group，model group，and traditional Chinese medicine (TCM) group，with 10 rats in each group.Permanent occlusion of both common carotid arteries was performed to establish a rat model of VaD，and after successful modeling，the rats in the TCM group were given Wenfei Jiangzhuo prescription by gavage，while those in the sham-operation group and the model group were given an equal volume of 0.9% sodium chloride injection by gavage，with a course of treatment of 4 weeks for all groups.The Morris water maze was used to assess learning and memory abilities；RT-PCR and Western blot were used to measure the mRNA and protein expression levels of Nogo-A and NgR；a transmission electron microscope was used to observe the change in neuronal synaptic structure.Results：Compared with the sham-operation group，the model group had a significant increase in escape latency and a significant reduction in the number of platform crossings (P<0.01 or P<0.05)，as well as significant increases in the protein and mRNA expression levels of Nogo-A and NgR in the hippocampus (P<0.01).Compared with the model group，the TCM group had a significant reduction in escape latency and a significant increase in the number of platform crossings (P<0.05)，as well as significant reductions in the protein and mRNA expression levels of Nogo-A and NgR in the hippocampus (P<0.01).Hippocampal neurons had complete synapses and organelles，with normal synaptic cleft and vesicle.Conclusion：Wenfei Jiangzhuo prescription can improve the learning and memory abilities of VaD rats，possibly by reducing the mRNA and protein expression levels of Nogo-A and NgR，promoting neuronal synapse regeneration，and improving hippocampal synaptic plasticity. |
| Key words: vascular dementia neuronal synaptic plasticity Wenfei Jiangzhuo prescription Nogo-A NgR experimental study |
