| 摘要: |
| 目的：采用双波长HPLC同时测定参橘草营养强化饮液中5种化合物(腺苷、虫草素、人参皂苷Rg1、人参皂苷Re、橙皮苷)的含量。方法:依利特Hypersil BDS C18色谱柱(4.6 mm×250 mm，5 μm)；流动相：乙腈-水，梯度洗脱；流速：1.0 ml/min；柱温25℃；检测波长203 nm(人参皂苷Rg1、人参皂苷Re)，260 nm(腺苷、虫草素、橙皮苷)。结果：5种化合物在各自的浓度范围内均呈现良好的线性关系(r>0.9990)，平均加样回收率（n=6）分别为96.72%、97.93%、98.04%、99.22%、101.69%。结论：该法简单快捷、稳定可靠，准确率高，重复性好，可用于参橘草营养强化饮液的质量评价。 |
| 关键词: 参橘草营养强化饮液 高效液相色谱法 腺苷 虫草素 橙皮苷 人参皂苷Rg1 人参皂苷Re |
| DOI： |
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| Simultaneous determination of five components in Shenjucao nutrition reinforcement liquid by dual-wavelength reverse-phase high-performance liquid chromatography |
| LU Maofang,LI Wei,LI Xiaolan |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China) |
| Abstract: |
| Objective：To establish the dual-wavelength high-performance liquid chromatography (HPLC) method for the simultaneous determination of five components (adenosine，cordycepin，ginsenoside Rg1，ginsenoside Re，and hesperidin) in Shenjucao nutrition reinforcement liquid.Methods：HPLC was performed on an Elite Hypersil BDS C18 column (4.6 mm × 250 mm，5 μm) with the mobile phase of acetonitrile-water for gradient elution at a flow rate of 1.0 ml/min，a column temperature of 25°C，a detection wavelength of 203 nm (for ginsenoside Rg1 and ginsenoside Re) or 260 nm (for adenosine，cordycepin，and hesperidin).Results：All five compounds showed a good linear relationship with the respective range of concentration (r>0.9990)，with an average recovery of 96.72%，97.93%，98.04%，99.22%，and 101.69%，respectively (n=6).Conclusion：This method is simple，rapid，stable，and reliable with high accuracy and good repeatability，and it can be used for the quality assessment of Shenjucao nutrition reinforcement liquid. |
| Key words: Shenjucao nutrition reinforcement liquid high-performance liquid chromatography adenosine cordycepin hesperidin ginsenoside Rg1 ginsenoside Re |
