| 摘要: |
| 目的：运用网络药理学方法探讨浙贝母治疗小儿腺样体肥大的作用机制。方法：应用 TCMSP平台及UniPort数据库检索并筛选浙贝母的活性成分及其对应的靶基因，通过GeneCards数据库获取腺样体肥大的靶点基因，并筛选浙贝母有效活性成分与腺样体肥大的交集靶点，使用Cytoscape 3.9.0软件绘制蛋白质-蛋白质相互作用（PPI）网络图，预测其关键靶点；对交集靶点进行基因本体（GO）功能和京都基因与基因组百科全书（KEGG）通路富集分析。结果：浙贝母治疗腺样体肥大的有效化合物包含天竺葵素、β-谷甾醇、贝母辛碱、浙贝树脂醇、6-甲氧基-2-乙酰基-3-甲基-1，4-萘醌-8-O-β-D-吡喃葡萄糖苷，相关潜在靶点涉及腺苷受体（AR）、过氧化物酶体增生激活受体γ（PPARG）、糖皮质激素受体（NR3C1）等。GO功能富集分析显示预测靶点主要与雌二醇的反应、药物反应、对脂多糖的反应、相同蛋白结合、凋亡过程的正调控等分子功能相关。KEGG通路富集分析显示潜在基因主要集中在凋亡-多物种、肿瘤蛋白p53、白细胞介素-17、肿瘤坏死因子（TNF）、血管内皮生长因子等信号通路。结论：浙贝母可能通过诱导凋亡、减轻炎症反应、调节免疫系统等多靶点、多通路发挥治疗腺样体肥大的作用。 |
| 关键词: 腺样体肥大 浙贝母 网络药理学 作用机制 |
| DOI： |
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| Mechanism of action of Fritillaria thunbergii in treatment of pediatric adenoid hypertrophy：A study based on network pharmacology |
| JIN Hanzhi,HAO Ruifang |
| (The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine & National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion，Tianjin 300193，China) |
| Abstract: |
| Objective：To investigate the mechanism of action of Fritillaria thunbergii in the treatment of pediatric adenoid hypertrophy based on network pharmacology.Methods：TCMSP platform and UniPort database were used to screen out the active components of Fritillaria thunbergii and their corresponding target genes，and GeneCards database was used to obtain the target genes of adenoid hypertrophy.Intersecting targets were obtained for the active components of Fritillaria thunbergii and adenoid hypertrophy，and Cytoscape 3.9.0 was used to plot a protein-protein interaction network and predict key targets.Gene ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed for the intersecting targets.Results：The active compounds of Fritillaria thunbergii in the treatment of adenoid hypertrophy included pelargonidin，beta-sitosterol，peimisine，zhebeiresinol，and 6-methoxyl-2-acetyl-3-methyl-1，4-naphthoquinone-8-O-β-D-glucopyranoside，and related potential targets involved adenosine receptor，peroxisome proliferator activated receptor γ，and glucocorticoid receptor NR3C1.GO functional enrichment analysis showed that the targets were mainly associated with the molecular functions such as estradiol response，drug response，response to lipopolysaccharide，binding of the same proteins，and positive regulation of apoptosis.KEGG pathway enrichment analysis showed that the potential genes were mainly involved in the signaling pathways such as apoptosis-multispecies，tumor protein p53，interleukin-17，tumor necrosis factor，and vascular endothelial growth factor.Conclusion：Fritillaria thunbergii may exert a therapeutic effect on adenoid hypertrophy by inducing apoptosis，alleviating inflammatory response，and regulating the immune system via multiple targets and pathways. |
| Key words: adenoid hypertrophy Fritillaria thunbergii network pharmacology mechanism of action |
