| 摘要: |
| 目的：运用DPPH-UPLC-Q-Exactive-MS法研究藤茶的抗氧化活性成分。方法：以不同炮制品的藤茶为对象，考察其清除 DPPH自由基的能力，并运用UPLC-Q-Exactive-MS技术对藤茶的抗氧化成分进行定性分析。结果：藤茶提取物对DPPH自由基清除能力强，具有较好的抗氧化活性，UPLC-Q-Exactive-MS分析共鉴定了18个化合物，其中黄酮类化合物15个。结论：应用 DPPH 抗氧化活性筛选及 UPLC-Q-Exactive-MS 技术能够快速准确地发现藤茶抗氧化活性成分，本研究结果可为进一步研究藤茶抗氧化的药效物质研究提供参考。 |
| 关键词: 藤茶 抗氧化活性 UPLC-Q-Exactive-MS |
| DOI： |
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| In vitro antioxidant activity of Ampelopsis megalophylla Diels et Gilg based on DPPH-UPLC-Q-Exactive-MS |
| WAN Dan,SHI Zhihua,SHEN Bingbing |
| (Hunan Academy of Chinese Medicine，Changsha 410013，Hunan，China;Huaihua First People’s Hospital，Huaihua 418000，Hunan，China) |
| Abstract: |
| Objective：To investigate the antioxidant active components of Ampelopsis megalophylla Diels et Gilg based on DPPH-UPLC-Q-Exactive-MS.Methods：The different processed products of Ampelopsis megalophylla Diels et Gilg were observed for the ability to scavenge free radicals，and the UPLC-Q-Exactive-MS technique was used to perform a qualitative analysis of the antioxidant components of Ampelopsis megalophylla Diels et Gilg.Results：The extract of Ampelopsis megalophylla Diels et Gilg had a strong ability to scavenge free radicals and showed a good antioxidant activity.A total of 18 compounds were identified by UPLC-Q-Exactive-MS，among which there were 15 flavonoids.Conclusion：The DPPH screening method for antioxidant activity and the UPLC-Q-Exactive-MS technique can be used to identify the active antioxidant components of Ampelopsis megalophylla Diels et Gilg，which provides a reference for further research on the material basis for the antioxidant effect of Ampelopsis megalophylla Diels et Gilg. |
| Key words: Ampelopsis megalophylla Diels et Gilg antioxidant activity UPLC-Q-Exactive-MS |
