| 摘要: |
| 目的：基于网络药理学方法探讨黄芪治疗肝纤维化的作用机制。方法：使用TCMSP、PubChem和Swiss Target Prediction数据库预测黄芪有效成分的靶点；通过GenCards数据库收集肝纤维化靶点，取交集得到黄芪对肝纤维化的作用靶点。运用Cytoscape 3.8.2构建黄芪-有效成分-靶点-肝纤维化网络，预测核心靶点，构建基于STRING平台的PPI网络。利用 Metascape平台进行基因本体（GO）功能和京都基因与基因组百科全书（KEGG）通路富集分析。结果：筛选得到黄芪有效成分20种，治疗肝纤维化的相关靶点90个，疗效明显的有效成分是山柰酚、华良姜素、异鼠李素、槲皮素等，核心靶点是白细胞介素-6（IL-6）、丝氨酸/苏氨酸激酶1（AKT1）、转录因子（JUN）、表皮生长因子受体（EGFR）、半胱氨酰天冬氨酸特异性蛋白酶3（CASP3）等。黄芪治疗肝纤维化途径主要包括癌症通路、EGFR酪氨酸激酶抑制剂抗通路、PI3K-Akt信号通路、乙型肝炎、丙型肝炎等信号通路。结论：通过网络药理学方法初步探讨黄芪治疗肝纤维化的作用机制，表明黄芪具有多成分、多靶点、多通路等作用特点，可为基础和临床研究提供参考依据。 |
| 关键词: 肝纤维化 黄芪 靶点预测 作用机制 网络药理学 |
| DOI： |
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| Mechanism of action of Astragalus membranaceus in treatment of liver fibrosis：A study based on network pharmacology |
| XU Jiying,LI Guizhen,LOU Yan |
| (Hubei University of Chinese Medicine，Wuhan 430065，Hubei，China) |
| Abstract: |
| Objective：To investigate the mechanism of action of Astragalus membranaceus in the treatment of liver fibrosis based on network pharmacology.Methods：TCMSP，PubChem，and Swiss Target Prediction databases were used to predict the targets of the effective constituents of Astragalus membranaceus，and GenCards database was used to obtain the targets of liver fibrosis；intersection of the two groups of targets was performed to obtain the action targets of Astragalus membranaceus on liver fibrosis.Cytoscape 3.8.2 was used to construct an Astragalus membranaceus-effective constituent-target-liver fibrosis network to predict core targets，and a protein-protein interaction network was constructed based on STRING platform.Metascape platform was used to perform gene ontology and Kyoto Encyclopedia of Genes and Genome pathway enrichment analyses.Results：A total of 20 effective constituents of Astragalus membranaceus was obtained，as well as 90 targets associated with the treatment of liver fibrosis；the effective constituents with a marked effect included kaempferol，kumatakenin，isorhamnetin，and quercetin，and the core targets included interleukin-6，AKT1，JUN，epithelial growth factor receptor (EGFR)，and CASP3.The main pathways involved in the treatment of liver fibrosis by Astragalus membranaceus mainly included cancer pathway，EGFR tyrosine kinase inhibitor pathway，the PI3K-Akt signaling pathway，the hepatitis B signaling pathway，and the hepatitis C signaling pathway.Conclusion：The mechanism of action of Astragalus membranaceus in the treatment of liver fibrosis preliminarily explored by network pharmacology shows that Astragalus membranaceus has the characteristics of multiple constituents，targets，and pathways，which provides a reference for basic and clinical research. |
| Key words: liver fibrosis Astragalus membranaceus target prediction mechanism of action network pharmacology |
