| 摘要: |
| 目的：通过体外建立大鼠心肌细胞缺氧复氧（HR）损伤模型，观察加味丹参饮（JWDS）能否通过激活Parkin蛋白介导的线粒体自噬而发挥对缺血再灌注心肌的保护作用。方法：将H9C2心肌细胞传代培养后，随机分为5组，分别为正常对照组（NG）、模型组（HRG）、模型组+空白血清处理组（BSG）、模型组+加味丹参饮含药血清组（JWDSG）、模型组+加味丹参饮含药血清+Mdivi-1组（JDMG），应用免疫印迹法比较各组心肌细胞内Parkin蛋白、Beclin-1蛋白的表达情况。结果：与NG组比较，HRG、BSG、JDMG、JWDSG的Parkin、Beclin-1蛋白表达均有所升高，差异均有统计学意义（P<0.05）；与BSG、JDMG组比较，JWDSG的Parkin、Beclin-1蛋白表达升高，差异均有统计学意义（P<0.05）；HRG、BSG、JDMG 3组之间，以上2项指标比较，差异均无统计学意义（P>0.05）。结论：加味丹参饮可能通过调控Parkin蛋白通路，激活线粒体自噬相关蛋白，发挥减轻心肌缺血再灌注损伤的作用。 |
| 关键词: 缺氧复氧损伤 加味丹参饮 线粒体自噬 Parkin通路 实验研究 |
| DOI： |
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| Effect of Jiawei Danshen decoction on mitophagy in cardiomyocytes with hypoxia/reoxygenation injury：A study based on the Parkin signaling pathway |
| YANG Yanhong,LI Li,WU Ruoxia |
| (College of Traditional Chinese Medicine，Hunan University of Chinese Medicine，Changsha 410208，Hunan，China) |
| Abstract: |
| Objective：To establish a rat cardiomyocyte model of hypoxia/reoxygenation injury in vitro，and to investigate whether Jiawei Danshen decoction (JWDS) can exert a protective effect against hypoxia/reoxygenation injury in myocardium by activating mitophagy mediated by Parkin protein.Methods：H9C2 cardiomyocytes after subculture were randomly divided into normal control group (NG group)，model group (HRG) group，model+blank serum treatment group (BSG group)，model+serum containing JWDS group (JWDSG group)，and model+serum containing JWDS+Mdivi-1 group (JDMG group)，and Western blotting was used to compare the protein expression of Parkin and Beclin-1 in cardiomyocytes between groups.Results：Compared with the NG group，the HRG，BSG，JDMG，and JWDSG groups had significant increases in the protein expression of Parkin and Beclin-1 (P<0.05)，and compared with the BSG and JDMG groups，the JWDSG group had significant increases in the protein expression of Parkin and Beclin-1 (P<0.05)，while there were no significant differences in these indices between the HRG，BSG，and JDMG groups (P>0.05).Conclusion：JWDS can alleviate ischemia/reoxygenation injury in myocardium by regulating the Parkin pathway and activating mitophagy-related proteins. |
| Key words: hypoxia/reoxygenation injury Jiawei Danshen decoction mitophagy Parkin pathway experimental study |
