| 摘要: |
| 目的：运用网络药理学方法研究苗药杜仙健骨方治疗膝骨关节炎(KOA)并骨质疏松症（OP）的生物活性成分和潜在作用机制。方法：运用中药系统药理学数据库和分析平台（TCMSP）,根据苗药所对应的中药,筛选出杜仙健骨方全方的主要活性成分及其相关作用靶标,借助OMIM、Drugbank、Genecards数据库,筛选出药物-疾病的共同作用靶标,并通过Venny 2.1.0绘制韦恩（Venn）图,利用Cytoscape软件绘制出药物-活性成分-靶点-疾病调控网络,将数据输入String数据库构建蛋白互作（PPl）网络和条形图,筛选核心靶标。最后通过Metascape软件进行基因本体（GO）功能及京都基因与基因组百科全书（KEGG）通路富集分析。结果：杜仙健骨方中发挥治疗KOA并OP作用的主要有效成分有槲皮素(Quercetin)、山柰酚(Kaempferol)、木犀草素(Luteolin)、β-谷甾醇(Beta-sitosterol)、黄芩素(Baicalein)等66个;关键靶基因230个,包括肿瘤坏死因子(TNF)、蛋白激酶B(AKT1)、癌基因(JUN)等;GO富集分析得到1266个生物学过程;KEGG通路分析明确了366条信号通路。结论：杜仙健骨方主要通过糖尿病并发症中的AGE-RAGE信号通路、癌症的途径、膀胱癌、破骨细胞分化的信号通路等的特点,调节细胞增殖-分化-凋亡过程,发挥抑制骨质疏松、控制炎症等作用,从而达到治疗KOA并OP的效果。 |
| 关键词: 松仙健骨方 膝骨关节炎 骨质疏松症 网络药理学 信号通路 |
| DOI： |
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| Mechanism of action of the Miao medicine Duxian Jiangu prescription in treatment of knee osteoarthritis with osteoporosis：A study based on network pharmacology |
| ZHANG Kaiwei,SHEN Jun |
| (The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang 550001,Guizhou,China) |
| Abstract: |
| Objective：To investigate the bioactive components and potential mechanism of action of the Miao medicine Duxian Jiangu prescription in the treatment of knee osteoarthritis (KOA) with osteoporosis (OP) based on network pharmacology.Methods：Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform and the corresponding traditional Chinese medicine drugs of Miao medicine were used to obtain the main active components of the whole prescription of Duxian Jiangu prescription and their action targets,and OMIM,Drugbank,and Genecards databases were used to screen out the common targets of drug and disease.Venny 2.1.0 was used to plot a Venn diagram,and Cytoscape software was used to establish a drug-active component-target-disease regulatory network.Related data were entered into String database to establish protein-protein interaction network and bar charts and screen out core targets.Metascape software was used to perform gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses.Results：A total of 66 main effective constituents of Duxian Jiangu prescription exerted a therapeutic effect on KOA with OP,including quercetin,kaempferol,luteolin,beta-sitosterol,and baicalein,and there were 230 key target genes,including TNF,AKT1,and JUN.GO enrichment analysis obtained 1266 biological processes,and KEGG pathway analysis clarified 366 signaling pathways.Conclusion：Duxian Jiangu prescription regulates the cell proliferation-differentiation-apoptosis process,inhibits OP,and controls inflammation through the pathways such as the AGE-RAGE signaling pathway in diabetic complication,cancer pathway,bladder cancer,and osteoclast differentiation,thereby exerting a therapeutic effect on KOA with OP. |
| Key words: Duxian Jiangu prescription knee osteoarthritis osteoporosis network pharmacology signaling pathway |
