| 摘要: |
| 目的：探讨木瓜蛋白酶联合运动装置建立膝关节创伤性滑膜炎动物模型的方法。方法：将10只新西兰兔随机分为空白组和模型组，每组各5只。从造模实验开始的第1、4、7天将0.1 ml 4%的木瓜蛋白酶水溶液注射于模型组兔的右后肢关节腔内，第8天开始，将模型组麻醉后，将其四肢固定于运动装置上继续造模，共造模7 d。测量兔造模肢膝关节周径变化，并观察其跛行情况。造模结束后，取右后肢膝关节腔积液、滑膜、软骨及周围肌肉，检测白细胞介素-1（IL-1β）、肿瘤坏死因子-α（TNF-α）的水平；观察滑膜、软骨、肌肉的病理组织情况。结果：2组关节周径及膝关节积液，滑膜，肌肉中IL-1β、TNF-α比较，差异均有统计学意义（P<0.05）；滑膜、软骨、肌肉组织的病理结果显示炎性改变。结论：运动结合木瓜蛋白酶完善了单一使用木瓜蛋白酶建立膝关节创伤性滑膜炎模型的不足，造模成功且效果好。 |
| 关键词: 创伤性滑膜炎 膝关节 运动 木瓜蛋白酶 动物模型 |
| DOI： |
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| Establishment of a rabbit model of traumatic knee synovitis by papain combined with an exercise device：An experimental study |
| PEI Xin,LIU Mengjiao,SUN Qiaoyan |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China) |
| Abstract: |
| Objective：To investigate the method for establishing an animal model of traumatic knee synovitis by papain combined with an exercise device.Methods：A total of 10 New Zealand rabbits were randomly divided into blank group and model group，with 5 rabbits in each group.On days 1，4，and 7 after the modeling experiment started，0.1 ml 4% papain solution was injected in to the articular cavity of the right hindlimb，and since day 8，the rabbits in the model group were anesthetized with the limbs fixed on the exercise device for modeling for another 7 days.The change in the circumference of the knee joint for modeling was measured，and the condition of claudication was observed.After modeling，effusion，synovial membrane，cartilage，and surrounding muscles of the knee joint of the right hindlimb were collected to measure the levels of interleukin-1β （IL-1β） and tumor necrosis factor-α （TNF-α） and observe the pathological changes of synovial membrane，cartilage，and muscle.Results：There were significant differences between the two groups in the circumference of the knee joint and the levels of IL-1β and TNF-α in the effusion，synovial membrane，and muscle of the knee joint （P<0.05）.Pathological results of synovial membrane，cartilage，and muscle tissue showed inflammatory change.Conclusion：Exercise combined with papain improves the shortcoming of the model of traumatic knee synovitis established by papain alone and can successfully establish the model. |
| Key words: traumatic synovitis knee joint exercise papain animal model |
