| 摘要: |
| 目的：制备卵巢过度刺激综合征(OHSS)倾向多囊卵巢综合征(PCOS)大鼠模型,并对造模方法进行评价。方法：将SD雌性幼鼠60只分为实验组（48只）和空白对照组（12只）。实验组大鼠予颈背部皮下注射丙酸睾丸酮+中性茶油进行PCOS造模,空白对照组注射中性茶油,连续20 d。造模成功的41只大鼠随机分为PCOS对照组10只、高剂量人绒毛膜促性腺激素（hCG）模型组（简称高hCG组）11只、中剂量hCG模型组（简称中hCG组）10只、低剂量hCG模型组（简称低hCG组）10只。高、中、低hCG组分别给予颈背部皮下注射高、中、低剂量hCG+0.9%氯化钠注射液干预,空白对照组和PCOS对照组注射0.9%氯化钠注射液,连续9 d。比较各组大鼠体质量、卵巢病理形态学改变、卵巢指数、卵巢最大横截面积、腹水评分、腹腔伊文思蓝（EB）含量、血清雌二醇（E2）水平。结果：模型组大鼠卵巢体积增大,卵巢指数降低,最大横截面积增大,卵巢出现多发性滤泡囊肿和黄体囊肿;E2水平升高;腹腔毛细血管通透性增加。与空白对照组相比,高hCG组各项指标差异均有统计学意义（P<0.05）;中hCG组除腹水评分外,其余各项指标差异均有统计学意义（P<0.05）;低hCG组除腹水评分、卵巢指数外,其余各项指标差异均有统计学意义（P<0.05）。与PCOS对照组相比,高、中hCG组大鼠卵巢指数、卵巢最大横截面积、腹腔EB含量、血清E2水平差异均有统计学意义（P<0.05）;低hCG组卵巢指数、卵巢最大横截面积、血清E2水平差异均有统计学意义（P<0.05）。结论：丙酸睾丸酮联合中剂量hCG可以诱导OHSS倾向PCOS大鼠模型,该模型的卵巢病理形态学改变、腹腔毛细血管通透性增加、血清E2水平明显升高,但未出现明显腹水,接近人类OHSS倾向PCOS患者的改变。 |
| 关键词: 卵巢过度刺激征 多囊卵巢综合征 丙酸睾丸酮 人绒毛膜促性腺激素 |
| DOI： |
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| Establishment and evaluation of a rat model of polycystic ovary syndrome with the tendency of ovarian hyperstimulation syndrome induced by testosterone propionate and human chorionic gonadotrophin |
| LIU Xia,TANG Zhiming,YANG Meichun |
| (Zhaoqing Medical College,Zhaoqing 526020,Guangdong,China;Guangxi University of Chinese Medicine,Nanning 530001,Guangxi,China) |
| Abstract: |
| Objective：To establish a rat model of polycystic ovary syndrome (PCOS) with the tendency of ovarian hyperstimulation syndrome (OHSS),and to evaluate the modeling method.Methods：A total of 60 female young Sprague-Dawley rats were divided into experimental group with 48 rats and blank control group with 12 rats.The rats in the experimental group were given subcutaneous injection of testosterone propionate and neutral tea oil at the neck and back to establish a model of PCOS,and those in the blank control group were given the injection of neutral tea oil,for 20 consecutive days.After successful modeling,41 rats were randomly divided into PCOS control group with 10 rats,high-dose human chorionic gonadotrophin (hCG) model group (high-dose hCG group) with 11 rats,middle-dose hCG model group (middle-dose hCG group) with 10 rats,and low-dose hCG model group (low-dose hCG group) with 10 rats.The high-,middle-,and low-dose hCG groups were given subcutaneous injection of 0.9% sodium chloride injection and high-,middle-,and low-dose hCG,respectively,at the neck and back,and the blank control group and the PCOS group were given injection of 0.9% sodium chloride injection,for 9 consecutive days.The groups were compared in terms of body weight,pathomorphological changes of the ovary,ovary index,maximum cross-sectional area of the ovary,ascites score,content of Evans blue (EB) in the abdomen,and the serum level of estradiol (E2).Results：The rats in the model group had increases in ovarian volume,maximum cross-sectional area of the ovary,E2 level,and capillary permeability in the abdomen and a reduction in ovary index,with the presence of multiple follicular cysts and corpus luteum cysts in the ovary.There were significant differences in all indices between the blank control group and the high-dose hCG group (P<0.05);there were significant differences in all indices except ascites score between the blank control group and the middle-dose hCG group (P<0.05);there were significant differences in all indices except ascites score and ovary index between the blank control group and the low-dose hCG group (P<0.05).There were significant differences in ovary index,maximum cross-sectional area of the ovary,EB content in the abdomen,and the serum level of E2 between the high- and middle-dose hCG groups and the PCOS control group (P<0.05),and there were significant differences in ovary index,maximum cross-sectional area of the ovary,and the serum level of E2 between the low-dose hCG group and the PCOS control group (P<0.05).Conclusion：Testosterone propionate combined with middle-dose hCG and induce a rat model of PCOS with OHSS tendency,and this model shows pathomorphological changes of the ovary,increased capillary permeability in the abdomen,and a significant increase in the serum level of E2,without the presence of marked ascites,which are similar to the changes in patients with PCOS with OHSS tendency. |
| Key words: ovarian hyperstimulation syndrome polycystic ovary syndrome testosterone propionate human chorionic gonadotrophin |
