| 摘要: |
| 目的：探讨蛭龙活血通瘀胶囊(以下简称“ZL”)对H2O2诱导细胞氧化损伤的保护作用。方法：以正常H9C2心肌细胞、PC12细胞作为对照，分别以不同浓度的H2O2处理细胞4 h，建立氧化损伤模型;在H2O2处理细胞前用ZL预处理24 h，作为ZL药物组，采用CCK-8法检测以上各组细胞存活率。结果：与未经H2O2处理的细胞比较，400 μmol/L H2O2处理的H9C2心肌细胞存活率下降至(47.25±4.14)%（P<0.01）;600 μmol/L H2O2处理的PC12细胞存活率下降至(52.53±4.31)%（P<0.01）;经10～320 mg/ml的ZL预处理后，H9C2心肌细胞、PC12细胞的存活率均高于模型组，差异有统计学意义（P<0.05）。结论：ZL对H2O2诱导的H9C2心肌细胞、PC12细胞氧化损伤有保护效果。 |
| 关键词: 蛭龙活血通瘀胶囊 H2O2诱导的氧化损伤 H9C2心肌细胞 PC12细胞 |
| DOI： |
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| Protective effect of Zhilong Huoxue Tongyu capsules against H2O2-induced oxidative injury in cells |
| TANG Run,ZHANG Juan,MA Yue |
| (Southwest Medical University，Luzhou 646000，Sichuan，China;The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University，Luzhou 646000，Sichuan，China) |
| Abstract: |
| Objective：To investigate the protective effect of Zhilong Huoxue Tongyu capsules (hereinafter referred to as ZL) against H2O2-induced oxidative injury in cells. Methods：With normal H9C2 cardiomyocytes and PC12 cells as a control，the cells were treated with different concentrations of H2O2 for 4 hours to establish a model of oxidative injury. The cells pretreated with ZL for 24 hours before H2O2 treatment were established as ZL group，and CCK-8 assay was used to measure cell viability in each group. Results：Compared with the cells not treated by H2O2，H9C2 cardiomyocytes treated with 400 μmol/L H2O2 had the cell viability reduced to 47.25%±4.14%(P<0.01)，and PC12 cells treated with 600 μmol/L H2O2 had the cell viability reduced to 52.53%±4.31% (P<0.01). The H9C2 cardiomyocytes and PC12 cells pretreated with 10～320 mg/ml ZL had significantly higher cell viability than the model group (P<0.05). Conclusion：ZL exerts a protective effect against H2O2-induced oxidative injury in H9C2 cardiomyocytes and PC12 cells. |
| Key words: Zhilong Huoxue Tongyu capsules H2O2-induced oxidative injury H9C2 cardiomyocyte PC12 cell |
