| 摘要: |
| 目的：建立中药舒心片中丹酚酸B含量的测定方法。方法：采用高效液相色谱法（HPLC）测定舒心片中丹酚酸B的含量,色谱柱为Agilent TC-C18（2）色谱柱（4.6 mm×250 mm,5 μm）,以乙腈-0.1%的磷酸溶液（25∶75）为流动相;检测波长为286 nm;柱温为25℃;流速为1.0 ml/min。结果：供试品和对照品溶液中丹酚酸B与他峰分离度良好;在0.050～1.642 μg之间丹酚酸B呈良好线性关系,R=0.9993,丹酚酸B平均加样回收率为97.58%,RSD=0.25%。结论：经方法学验证,本法可用于舒心片的质量控制。 |
| 关键词: HPLC 舒心片 丹酚酸B 含量测定 |
| DOI： |
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| A study of quality standard for Shuxin tablets |
| LIU Mingzhi,LIANG Hong,HAO Xu |
| (Pingxiang Institute for Food and Drug Control,Pingxiang 337000,Jiangxi,China;Pingxiang Hospital of Traditional Chinese Medicine,Pingxiang 337000,Jiangxi,China) |
| Abstract: |
| Objective：To establish a method for the content determination of salvianolic acid B in the traditional Chinese medicine Shuxin tablets.Methods：To determine the content of salvianolic acid B in Shuxin tablets,high-performance liquid chromatography was performed on an Agilent TC-C1818(2) column (4.6 mm×250 mm,5 μm) with a mobile phase of acetonitrile-0.1% phosphoric acid solution (25∶75),at a detection wavelength of 286 nm,a column temperature of 25℃,and a flow rate of 1.0 ml/min.Results：The peak of salvianolic acid B was well separated from the other peaks in the solutions of the test article and the reference substance.Salvianolic acid B showed a good linear relationship within the range of 0.050～1.642 μg (R=0.9993),and the average recovery rate of salvianolic acid B was 97.58%,with a relative standard deviation of 0.25%.Conclusion：Methodological verification shows that this method can be used for the quality control of Shuxin tablets. |
| Key words: high-performance liquid chromatography Shuxin tablets salvianolic acid B content determination |
