| 摘要: |
| 目的：探讨健脾柔肝息风汤对抽动障碍模型大鼠运动性抽动的调节机制,为临床治疗抽动障碍提供实验依据。方法：将60只SD大鼠随机分为空白组,模型组,氟哌啶醇组,健脾柔肝息风汤低、中、高剂量组,每组各10只,除空白组外各组大鼠连续7 d腹腔注射亚氨基二丙腈（IDPN）造模,造模成功后连续灌胃6周,灌胃后第1、3、6周分别观察大鼠的行为学改变,采用RT-PCR法检测大鼠脑组织DRD1 mRNA、DRD2 mRNA含量。结果：灌胃6周后,与模型组比较,健脾柔肝息风汤中、高剂量组及氟哌啶醇组在运动行为评分、刻板行为评分及DRD1 mRNA含量方面均有所下降（P<0.05）;而健脾柔肝息风汤低、中、高剂量组及氟哌啶醇组在DRD2 mRNA含量方面有所下降（P<0.05）。结论：健脾柔肝息风汤可通过降低TD模型大鼠脑组织多巴胺DRD1 mRNA、DRD2 mRNA的含量,而改善TD模型大鼠运动性抽动行为。 |
| 关键词: 抽动障碍 中医药疗法 健脾柔肝息风汤 大鼠行为学 多巴胺受体 实验研究 |
| DOI： |
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| Effect of Jianpi Rougan Xifeng decoction on behavioristics and the mRNA expression of dopamine receptor in a rat model of tic disorder |
| ZHU Qinquan,CHEN Pan,ZENG Qingsong |
| (The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,Hunan,China;Zhongshan Hospital of Traditional Chinese Medicine,Zhongshan 528400,Guangdong,China;Guangxi International Zhuang Medicine Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning 530201,Guangxi,China) |
| Abstract: |
| Objective：To investigate the regulatory mechanism of Jianpi Rougan Xifeng decoction on motor tics in a rat model of tic disorder,and to provide an experimental basis for the clinical treatment of tic disorder.Methods：A total of 60 Sprague-Dawley rats were randomly divided into blank group,model group,haloperidol group,and low-,middle-,and high-dose Jianpi Rougan Xifeng decoction groups,with 10 rats in each group.All rats except those in the blank group were given iminodipropionitrile for 7 consecutive days to establish a model of tic disorder,and the drugs were given by gavage for 6 consecutive weeks after successful modeling.Behavioral changes were observed at weeks 1,3,and 6 of gavage,and RT-PCR was used to measure the mRNA expression levels of DRD1 and DRD2 in brain tissue.Results：Compared with the model group after 6 weeks of gavage,the middle- and high-dose Jianpi Rougan Xifeng decoction groups and the haloperidol group had significant reductions in motor behavior score,stereotyped behavior score,and mRNA expression of DRD1 (P<0.05),and the low-,middle-,and high-dose Jianpi Rougan Xifeng decoction groups and the haloperidol group had a significant reduction in the mRNA expression of DRD2 (P<0.05).Conclusion：Jianpi Rougan Xifeng decoction can improve motor tics in a rat model of tic disorder by reducing the mRNA expression of DRD1 and DRD2 in brain tissue. |
| Key words: tic disorder traditional Chinese medicine treatment Jianpi Rougan Xifeng decoction rat behavioristics dopamine receptor experimental study |
