| 摘要: |
| 目的：探讨丹莪内异方对子宫内膜异位症大鼠的治疗效果。方法：将60只大鼠随机分为假手术组，模型组，丹莪内异方高、中、低剂量组（灌胃剂量中分别含生药15、7.5、3.75 g/kg），阳性药达那唑胶囊组（阳性组，灌胃剂量为36 mg/kg），每组各10只。行自体子宫内膜移植术制备大鼠子宫内膜异位症模型，给药4周。采用光学显微镜观察异位内膜的病理组织学并评价其病变分级，采用ELISA法测定血清炎症细胞因子白介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的表达水平。结果：丹莪内异方可缩小子宫内膜异位症大鼠的异位内膜体积。与模型组相比，丹莪内异方各剂量组的异位内膜病变分级程度均减轻（P<0.01）。与假手术组比较，模型组血清IL-6和TNF-α水平均增高（P<0.01）；药物干预后，高、中、低剂量血清IL-6和TNF-α水平均较模型组降低，差异均有统计学意义（P<0.05或P<0.01）；低剂量组TNF-α与阳性组比较，差异有统计学意义（P＜0.05）。结论：丹莪内异方能有效缓解大鼠子宫内膜异位症的发生和发展，这可能与下调炎症细胞因子水平、抑制机体炎症反应等有关。 |
| 关键词: 子宫内膜异位症 丹莪内异方 大鼠 实验研究 |
| DOI： |
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| Study on the interventional effect and mechanism of Dan’e Neiyi prescription in treatment of rats with endometriosis |
| ZHANG Yangyang,LI Kun |
| (Beijing Armed Police Corps Hospital，Beijing 100027，China;School of Business and Economics，Beijing Normal University，Beijing 100875，China) |
| Abstract: |
| Objective：To investigate the effect of Dan’e Neiyi prescription in the treatment of rats with endometriosis.Methods：A total of 60 rats were randomly divided into sham-operation group，model group，high-，middle-，and low-dose Dan’e Neiyi prescription groups (treated with 15，7.5，and 3.75 g/kg crude drug，respectively)，and positive group (treated with the positive drug danazol capsules 36 mg/kg by gavage)，with 10 rats in each group.Autologous endometrial transplantation was performed to prepare a rat model of endometriosis，and the drugs were given for 4 weeks.A light microscope was used to observe the histopathology of the ectopic endometrium and evaluate the grade of lesion，and ELISA was used to measure the serum levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α).Results：Dan’e Neiyi prescription reduced the volume of the ectopic endometrium in rats with endometriosis.Compared with the model group，the three Dane Neiyi prescription groups had a reduction in the grade of lesion in the ectopic endometrium (P<0.01).Compared with the sham-operation group，the model group had significant increases in the serum levels of IL-6 and TNF-α (P<0.01)；compared with the model group after drug intervention，the high-，middle-，and low-dose groups had significant reductions in the serum levels of IL-6 and TNF-α (P<0.05 or P<0.01)；there was no significant difference in TNF-α between the low-dose group and the positive group (P<0.05).Conclusion：Dan’e Neiyi prescription can effectively alleviate the development and progression of endometriosis in rats，possibly by downregulating the levels of inflammatory cytokines and inhibiting inflammatory response. |
| Key words: endometriosis Dan’e Neiyi prescription rat experimental study |
