| 摘要: |
| 目的：观察痛泻要方对肝郁脾虚型肠易激综合征（IBS-D）大鼠肠道菌群的影响，基于“脑肠菌轴”角度探讨痛泻要方治疗IBS-D的作用机制。方法：将24只SD大鼠随机分为4组：即空白组（NormalG)、模型组(ModelG)、双歧杆菌三联活菌片组(ShuangG)、痛泻要方组（TongG)，每组各6只。通过夹尾结合番泻叶灌胃的方法制备肝郁脾虚型IBS-D大鼠模型，分别给予相应药物灌胃，NormalG以及ModelG予0.9%氯化钠注射液灌胃。采用16srDNA测序法分析IBS-D大鼠肠道菌群在治疗前后的变化。结果：在门水平上，TongG较ModelG增加了厚壁菌门、拟杆菌门的种群丰度，有效降低了含较多致病菌的变形菌门丰度；在纲水平上，TongG较ModelG增加了梭状芽胞杆菌纲、拟杆菌纲、杆菌纲的丰度，降低了丙型变形菌纲丰度；在目水平上，TongG较ModelG增加了梭菌目、乳杆菌目、拟杆菌目的丰度，降低了黄色单胞菌目、肠杆菌目丰度；在属水平上，TongG较ModelG增加了巨单胞菌属、劳特氏菌属种群丰度，有效降低了寡养单胞菌属丰度；韦恩图和花瓣图显示，NormalG菌群数量最多，ModelG最少，TongG较ModelG增多且程度大于ShuangG，共有种群数量更趋近于NormalG；稀释曲线和等级聚类曲线结果均显示TongG的肠道菌群丰度及均匀度更趋近NormalG；箱型图展示TongG与NormalG多样性、离散程度、中位数更接近；PCOA分析结果显示TongG相较ShuangG，与NormalG重合度更高。结论：痛泻要方能纠正IBS-D患者存在的菌群失衡，促使肠道菌群结构趋于正常，且改善程度优于双歧杆菌三联活菌片。 |
| 关键词: 肠易激综合征 痛泻要方 脑肠菌轴 肠道菌群 实验研究 |
| DOI： |
|
|
| Effect of Tongxieyaofang on gut microbiota in rats with diarrhea-predominant irritable bowel syndrome with liver depression and spleen deficiency |
| ZHANG Jianfeng,CHEN Jie,ZHAO Shuangmei |
| (Tianjin University of Traditional Chinese Medicine，Tianjin 300193，China;The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine，Tianjin 300250，China) |
| Abstract: |
| Objective：To investigate the effect of Tongxieyaofang on gut microbiota in rats with diarrhea-predominant irritable bowel syndrome (IBS-D) with liver depression and spleen deficiency and the mechanism of action of Tongxieyaofang in the treatment of IBS-D from the aspect of “brain-gut-microbiota axis”.Methods：A total of 24 Sprague-Dawley rats were randomly divided into blank group (NormalG group)，model group (ModelG group)，live combined Bifidobacterium and Lactobacillus tablets group (ShuangG group)，and Tongxieyaofang group (TongG group)，with 6 rats in each group.The rats were treated with tail clipping combined with senna by gavage to establish a model of IBS-D with liver depression and spleen deficiency，and related drugs were given by gavage；the rats in the NormalG group and the ModelG group were given 0.9% sodium chloride injection by gavage.16srDNA sequencing was used to analyze the change in gut microbiota after treatment in IBS-D rats.Results：At the phylum level，compared with the ModelG group，the TongG group had significant increases in the abundance of Firmicutes and Bacteroidetes and a significant reduction in the abundance of Proteobacteria which contained many pathogens；at the class level，compared with the ModelG group，the TongG group had significant increases in the abundance of Clostridia，Bacteroidia，and Bacilli and a significant reduction in the abundance of Gammaproteobacteria；at the order level；compared with the ModelG group，the TongG group had significant increases in the abundance of Clostridiales，Lactobacillales，and Bacteroidales and significant reductions in the abundance of Xanthomonadales and Enterobacterales；at the genus level，compared with the ModelG group，the TongG group had significant increases in the abundance of Megamonas and Lauterella and a significant reduction in the abundance of Stenotrophomonas.Venn diagram and flower plot showed that the NormalG group had the highest number of flora and the ModelG group had the lowest number of flora，and the TongG group had a higher number than the ModelG group and a greater increase than the ShuangG group；the number of common populations was closer to that in the Normal G group.The rarefaction curve and the hierarchical clustering curve showed that the abundance and uniformity of gut microbiota in the TongG group were closer to those in the NormalG group；the box plot showed that the TongG group and the NormalG group had similar diversity，degree of dispersion，and median；the PCoA analysis showed that the TongG group had a higher overlap ratio than the ShuangG group and the NormalG group.Conclusion：Tongxieyaofang can correct the imbalance of gut microbiota and promote the normal recovery of the structure of gut microbiota in IBS-D patients，with better improvements than live combined Bifidobacterium and Lactobacillus tablets. |
| Key words: irritable bowel syndrome Tongxieyaofang brain-gut-microbiota axis gut microbiota experimental study |
