| 摘要: |
| 目的:正交试验优选猫爪草纳米乳喷雾剂的最佳提取工艺。方法:以总黄酮提取量和浸膏得率的综合评分为评价指标，在单因素试验基础上，采用L9(3）正交试验考察猫爪草醇提工艺的溶剂体积、乙醇浓度、提取时间及提取温度对提取工艺的影响。结果:最佳提取工艺为以75%乙醇为提取溶剂，提取温度60 ℃，第1 次8倍量1.5 h，第2 次7倍量1 h，共提取2 次。结论:在单因素试验基础上，通过正交试验优选的提取工艺经验证稳定、简便易行、预测性好，为猫爪草纳米乳喷雾剂的进一步研发奠定了基础。 |
| 关键词: 猫爪草纳米乳喷雾剂 总黄酮 浸膏得率 正交试验 |
| DOI： |
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| Optimization of extraction process for Ranunculi Ternati Radix nanoemulsion spray based on orthogonal test |
| ZENG Chunjiao,CHEN Linglong,LI Yuehui |
| (Xiangtan Medicine & Health Vocational College，Xiangtan 411000，Hunan，China;The Affiliated Hospital of Hunan Academy of Chinese Medicine，Changsha 410006，Hunan，China) |
| Abstract: |
| Objective：To optimize the extraction process for Ranunculi Ternati Radix nanoemulsion spray based on orthogonal test.Methods：With the extraction yield of total flavonoids and the extract yield as assessment indices，based on a univariate experiment，the L9(3) orthogonal test was used to evaluate the influence of solvent volume，alcohol concentration，extraction duration，and extraction temperature on the alcohol extraction process for Ranunculi Ternati Radix.Results：The optimal extraction process was as follows：75% alcohol as the extraction solvent，an extraction temperature of 60 ℃，and extraction twice，with 8 times amount for 1.5 hours followed by 7 times amount for 1 hour.Conclusion：The extraction process optimized based on univariate experiment and orthogonal test is stable，simple，and convenient with good predictability，which lays a foundation for further research and development of Ranunculi Ternati Radix nanoemulsion spray. |
| Key words: Ranunculi Ternati Radix nanoemulsion spray total flavonoids extract yield orthogonal test |
