| 摘要: |
| 目的：观察补益营卫方对衰老表皮结构蛋白K17的基因和蛋白水平的影响。方法：将3个月龄小鼠（年轻组）与14个月龄小鼠（老年组）表皮细胞消化分离出来，然后进行传代培养，其中14个月龄小鼠（老年组）的表皮细胞分为2组，一组用常规方法培养细胞，另一组用含2.5%的补益营卫方培养基进行培养，采用荧光定量RT-PCR法和Western Blotting法分别检测细胞K17mRNA表达和细胞K17蛋白表达。结果：与年轻组比较，老年组的K17mRNA和蛋白表达水平均升高（P<0.05）；经药物干预后小鼠表皮细胞中K17mRNA和蛋白水平较老年组明显降低（P<0.05）。结论：补益营卫方可通过抑制衰老表皮结构蛋白K17的表达，起到延缓皮肤衰老作用。 |
| 关键词: 衰老 补益营卫方 表皮结构 角蛋白17 实验研究 |
| DOI： |
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| Effect of Buyi Yingwei prescription on keratin 17 in senescent epidermal structure |
| JIAO Cuiqin,WANG Hao,ZHOU Xiaoping |
| (School of Traditional Chinese Medicine，Ningxia Medical University，Yinchuan 750004，Ningxia，China) |
| Abstract: |
| Objective：To investigate the effect of Buyi Yingwei prescription on the mRNA and protein expression of keratin 17 (K17) in senescent epidermal structure.Methods：Epidermal cells were isolated from mice aged 3 months (young group) and those aged 14 months (elderly group)，and then subculture was performed.The epidermal cells from the elderly group were divided into two groups；one group was cultured with conventional method，and the other group was cultured in the medium containing 2.5% Buyi Yingwei prescription.RT-qPCR and Western blotting were used to measure the mRNA and protein expression of K17 in cells.Results：Compared with the young group，the elderly group had significant increases in the mRNA and protein expression of K17 (P<0.05).In the elderly group，the cells with drug intervention had significant reductions in the mRNA and protein expression of K17 compared with those without intervention (P<0.05).Conclusion：Buyi Yingwei prescription delays skin aging by inhibiting the expression of K17 in senescent epidermal structure. |
| Key words: senescence Buyi Yingwei prescription epidermal structure keratin 17 experimental study |
