| 摘要: |
| 目的：观察健脾解毒活血汤对幽门螺旋杆菌相关性胃炎（HAG）脾胃湿热证模型小鼠血清白介素-1β（IL-1β）、胃泌素（GAS）及胃内组织Hp感染数的影响，为研制新型抗Hp感染的中药提供实验依据。方法：选取40只SPF级昆明小鼠为实验对象，将其随机分为空白组、模型组、西药组和中药组，每组各10只。采用Hp菌液灌胃以建立HAG脾胃湿热证小鼠模型，西药组予以奥美拉唑钠肠溶胶囊灌胃，中药组予以健脾解毒活血汤灌胃，均连续给药14d。观察比较各组小鼠血清IL-1β、GAS与胃内组织Hp感染情况。结果：模型组小鼠血清IL-1β、GAS的含量较空白组明显升高（P<0.01，P<0.05）；与模型组比较，中药组和西药组血清IL-1β显著降低（P<0.05，P<0.01），GAS水平2组无明显差异。模型组小鼠胃组织Hp感染数较空白组明显增加（P<0.01），中药组和西药组胃组织Hp感染数较模型组显著降低（P<0.05）。结论：健脾解毒活血汤对HAG脾胃湿热证模型小鼠血清IL-1β及Hp感染数有明显改善作用，但对GAS影响不大，在一定程度上可有效治疗脾胃湿热型HAG。 |
| 关键词: 胃炎 幽门螺杆菌 脾胃湿热证 健脾解毒活血汤 IL-1β GAS 实验研究 |
| DOI： |
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| Effect of Jianpi Jiedu Huoxue decoction on interleukin-1β and gastrin in mice with Helicobacter pylori-associated gastritis with spleen-stomach damp-heat syndrome |
| HU Jinyang,JIANG Shisheng,XIAO Meiying |
| (The Affiliated Hospital of Hunan Academy of Chinese Medicine，Changsha 410006，Hunan，China;Institute of Chinese Materia Medica，Hunan Academy of Chinese Medicine，Changsha 410013，Hunan，China) |
| Abstract: |
| Objective：To investigate the effect of Jianpi Jiedu Huoxue decoction on the serum levels of interleukin-1β (IL-1β) and gastrin (GAS) and the number of cells with Helicobacter pylori (Hp) infection in gastric tissue in mice with Hp-associated gastritis (HAG) with spleen-stomach damp-heat syndrome，and to provide an experimental basis for the development of new traditional Chinese medicine (TCM) drugs for Hp infection.Methods：A total of 40 specific pathogen-free Kunming mice were selected and randomly divided into blank group，model group，Western medicine group，and TCM group，with 10 mice in each group.A mouse model of HAG with spleen-stomach damp-heat syndrome was established by Hp bacterial solution by gavage.The mice in the Western medicine group were given omeprazole sodium enteric-coated capsules by gavage，and those in the TCM group were given Jianpi Jiedu Huoxue decoction by gavage；both groups were treated for 14 days.The serum levels of IL-1β and GAS and Hp infection in gastric tissue were compared between groups.Results：The model group had significantly higher serum levels of IL-1β and GAS than the blank group (P<0.01，P<0.05)；compared with the model group，the TCM group and the Western medicine group had a significant reduction in the serum level of IL-1β (P<0.05，P<0.01)，while there was no significant difference in the level of GAS.Compared with the blank group，the model group had a significant increase in the number of cells with Hp infection in gastric tissue (P<0.01)，and compared with the model group，the TCM group and the Western medicine group had a significant reduction in the number of cells with Hp infection in gastric tissue (P<0.05).Conclusion：Jianpi Jiedu Huoxue decoction can significantly improve serum IL-1β level and number of cells with Hp infection in mice with HAG with spleen-stomach damp-heat syndrome，with little effect on GAS，and thus exerts a certain therapeutic effect on HAG with spleen-stomach damp-heat syndrome. |
| Key words: gastritis Helicobacter pylori spleen-stomach damp-heat syndrome Jianpi Jiedu Huoxue decoction interleukin-1β gastrin experimental study |
