| 摘要: |
| 目的：探究心痛方对心肌梗死大鼠钙调蛋白依赖性蛋白激酶Ⅱ(CaMKⅡ)mRNA表达及钙离子（Ca2+）浓度的影响。方法：将50只SD大鼠随机分为5组，分别为假手术组、模型组、心痛方高剂量组、心痛方低剂量组、硫氮卓酮组，采用结扎冠状动脉的方法建立急性心肌梗死大鼠模型，运用RT-PCR法测定各组大鼠心肌组织CaMKⅡ mRNA的表达，激光扫描共聚显微镜法检测心肌组织内Ca2+浓度，HE染色法测定心肌梗死面积。结果：与模型组比较，心痛方高、低剂量组与硫氮卓酮组CaMKⅡ mRNA表达及Ca2+浓度均降低、心肌梗死面积缩小(P<0.01或P<0.05)；与心痛方低剂量组比较，心痛方高剂量组、硫氮卓酮组CaMKⅡ mRNA表达、Ca2+浓度均降低(P<0.01)，心痛方高剂量组心肌梗死面积缩小(P<0.05)；与硫氮卓酮组比较，心痛方高剂量组CaMKⅡ mRNA表达、Ca2+浓度均降低(P<0.01)，心痛方高、低剂量组心肌梗死面积均缩小(P<0.01或P<0.05)。结论：心痛方能下调急性心肌梗死大鼠CaMKⅡ mRNA的表达，降低胞内Ca2+浓度，从而拮抗钙超载对心肌细胞的损害，缩小心肌梗死面积。 |
| 关键词: 急性心肌梗死 心痛方 CaMKⅡ Ca+ 实验研究 |
| DOI： |
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| Effect of Xintong prescription on the mRNA expression of CaMKⅡ and Ca2+ concentration in rats with acute myocardial infarction |
| LIU Xuejia,FAN Jinru,CHEN Tong |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China;The First Affiliated Hospital of Hunan University of Chinese Medicine，Changsha 410007，Hunan，China) |
| Abstract: |
| Objective：To investigate the effect of Xintong prescription on the mRNA expression of CaMKⅡ and Ca2+ concentration in rats with acute myocardial infarction.Methods：A total of 50 Sprague-Dawley rats were randomly divided into sham-operation group，model group，high- and low-dose Xintong prescription groups，and diltiazem group.Coronary artery ligation was performed to establish a rat model of acute myocardial infarction.RT-PCR was used to measure the mRNA expression of CaMKⅡ in myocardial tissue，a laser scanning confocal microscope was used to measure the concentration of Ca2+ in tissue，and HE staining was used to measure myocardial infarct area.Results：Compared with the model group，the high- and low-dose Xintong prescription groups and the diltiazem group had significant reductions in the mRNA expression of CaMKⅡ，the concentration of Ca2+，and myocardial infarct area (P<0.01 or P<0.05).Compared with the low-dose Xintong prescription group，the high-dose Xintong prescription group and the diltiazem group had significant reductions in the mRNA expression of CaMKⅡ and the concentration of Ca2+ (P<0.01)，and the high-dose Xintong prescription group had a significant reduction in myocardial infarct area (P<0.05).Compared with the diltiazem group，the high-dose Xintong prescription group had significant reductions in the mRNA expression of CaMKⅡ and the concentration of Ca2+ (P<0.01)，and the high- and low-dose Xintong prescription groups had a significant reduction in myocardial infarct area (P<0.01 or P<0.05).Conclusion：In rats with acute myocardial infarction，Xintong prescription can reduce the mRNA expression of CaMKⅡ and the concentration of Ca2+，antagonize cardiomyocyte injury due to calcium overload，and reduce myocardial infarct area. |
| Key words: acute myocardial infarction Xintong prescription CaMKⅡ Ca2+ experimental study |
