| 摘要: |
| 目的：研究扭肚藤水煎液对金黄色葡萄球菌、枯草芽孢杆菌、大肠埃希氏菌的体外抗菌活性，为临床规范使用该药提供实验依据。方法：制备扭肚藤水煎液，采用牛津杯法初筛检测其抑菌作用，再分别应用液体培养基2倍稀释法和琼脂平板法检测其最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。结果：扭肚藤水煎液对金黄色葡萄球菌、枯草芽孢杆菌、大肠埃希氏菌均有较强的抑菌作用。扭肚藤提取物对金黄色葡萄球菌MIC为62.5mg/L，MBC为125mg/ml；对大肠埃希菌、枯草芽孢杆菌的MIC均为125mg/L，MBC均为250mg/ml。 |
| 关键词: 扭肚藤 抗菌活性 最低抑菌浓度 最低杀菌浓度 实验研究 |
| DOI： |
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| In vitro antimicrobial activity of Jasminum elongatum water decoction |
| LIU Rong,LIU Xixia,ZHOU Qiang |
| (Changsha Medical University，Changsha 410219，Hunan，China) |
| Abstract: |
| Objective：To investigate the in vitro antimicrobial activity of Jasminum elongatum water decoction against Staphylococcus aureus，Bacillus subtilis，and Escherichia coli，and to provide an experimental basis for standard use of this drug in clinical practice.Methods：Jasminum elongatum water decoction was prepared，and the Oxford cup method was used for primary determination of its antimicrobial effect.The two-fold liquid medium dilution method and the agar plate method were used to determine its minimal inhibitory concentration（MIC）and minimum bactericidal concentration（MBC）.Results：Jasminum elongatum water decoction had a strong antimicrobial effect against Staphylococcus aureus，Bacillus subtilis，and Escherichia coli.Jasminum elongatum extract had an MIC of 62.5mg/L and an MBC of 125mg/ml against Staphylococcus aureus and an MIC of 125mg/L and an MBC of 250mg/ml against Escherichia coli and Bacillus subtilis. |
| Key words: Jasminum elongatum antimicrobial effect minimal inhibitory concentration minimum bactericidal concentration experimental study |
