| 摘要: |
| 目的：观察心痛泰对动脉粥样硬化（AS）兔主动脉组织病理学改变和主动脉组织NF-κB表达的影响。方法：将120只新西兰大耳白兔随机分成6组，分别为正常对照组、模型组（均灌胃等体积0.9%氯化钠注射液），心痛泰低、中、高剂量组（分别灌胃心痛泰2.3g/kg、4.6g/kg、9.2g/kg），阿托伐他汀钙组（灌胃阿托伐他汀钙1.1mg/kg），每组20只。通过高脂饲料喂养及免疫反应损伤法制备兔AS模型，造模的同时给予药物干预。8周后，光学显微镜观察兔主动脉组织病理学改变；采用Western bolt法分别检测兔主动脉组织细胞核及细胞浆中NF-κB的表达；采用RT-PCR法检测兔主动脉组织NF-κB mRNA的表达。结果：与模型组比较，各用药组主动脉内膜厚度变薄，内膜下泡沫细胞及炎性细胞的浸润均有不同程度的减轻，主动脉NF-κB核转位及NF-κB mRNA明显降低（P<0.01或P<0.05）。结论：心痛泰对AS兔主动脉组织有一定的保护作用，通过下调AS兔主动脉组织NF-κB核转位和NF-κB mRNA表达，抑制炎症反应，从而延缓动脉粥样硬化的形成。 |
| 关键词: 动脉粥样硬化 心痛泰 炎症反应 NF-κB 实验研究 |
| DOI： |
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| Effect of Xintongtai on the expression of nuclear factor-kappa B in the aorta of rabbits with atherosclerosis |
| REN Xin,GUO Zhi-hua |
| (The First Affiliated Hospital of Hunan University of Chinese Medicine，Changsha 410007，Hunan，China;Hunan University of Chinese Medicine，Changsha 410208，Hunan，China) |
| Abstract: |
| Objective：To investigate the effect of Xintongtai on pathological changes of the aorta and the expression of nuclear factor-kappa B（NF-κB) in the aorta in rabbits with atherosclerosis（AS).Methods：A total of 120 New Zealand big-eared white rabbits were randomly divided into normal control group，model group（treated with an equal volume of 0.9% sodium chloride injection by gavage)，low-，middle-，and high-dose Xintongtai groups（treated with Xintongtai at a dose of 2.3，4.6，and 9.2 g/kg，respectively，by gavage)，and atorvastatin calcium group（treated with atorvastatin calcium at a dose of 1.1 mg/kg by gavage)，with 20 rabbits in each group.A rabbit model of AS was established by high-fat diet and immunoreactive injury，and drug intervention was given during modeling.After 8 weeks，a light microscope was used to observe the pathological changes of the aorta；Western blot was used to measure the expression of NF-κB in nuclei and cytoplasm in the aorta；RT-PCR was used to measure the mRNA expression of NF-κB in the aorta.Results：Compared with the model group，each administration group had a significant reduction in intimal thickness of the aorta，varying degrees of infiltration of foam cells and inflammatory cells in the intima，and significant reductions in nuclear translocation and mRNA expression of NF-κB in the aorta（P<0.01 or P<0.05).Conclusion：Xintongtai has a certain protective effect on the aorta of rabbits with AS and can delay the formation of AS by downregulating the nuclear translocation and mRNA expression of NF-κB and inhibiting inflammatory response. |
| Key words: atherosclerosis Xintongtai inflammatory response nuclear factor-kappa B experimental study |
