| 摘要: |
| 目的：研究脾胃培源灌肠方对UC大鼠δ阿片受体（DOR）、β-arrestin1、Bcl-2在结肠组织中表达的影响。方法：雄性SD健康大鼠120只，随机分为正常组，模型组，脾胃培源灌肠方大、中、小剂量组，美沙拉嗪组，每组20只。除正常组外，其余组均采用TNBS/乙醇法建立溃疡性结肠炎大鼠模型。正常组和模型组予0.9%氯化钠注射液3mL/d灌肠；美沙拉嗪组予美沙拉嗪悬浊液3mL/d灌肠；脾胃培源灌肠方大、中、小剂量组分别按含不同生药浓度（0.56g/L、0.28g/L、0.14g/L）的药液3mL/d灌肠，持续15d，第16天处死大鼠。采用免疫组织化学法和Real time-PCR检测大鼠结肠组织DOR、β-arrestin1、Bcl-2免疫阳性细胞表达及基因表达。结果：与正常组比较，模型组结肠组织DOR、β-arrestin1、Bcl-2免疫阳性细胞表达和mRNA表达显著升高（P<0.05）；与模型组比较，脾胃培源灌肠方大、中、小剂量组和美沙拉嗪组DOR、β-arrestin1、Bcl-2免疫阳性细胞表达和mRNA表达均降低（P<0.05）。结论：脾胃培源灌肠方通过对UC大鼠DOR、β-arrestin1、Bcl-2表达的影响从而发挥其治疗作用。 |
| 关键词: 溃疡性结肠炎 大鼠 DOR β-arrestin 1 Bcl-2 脾胃培源灌肠方 实验研究 |
| DOI： |
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| Effect of Piwei Peiyuan enema prescription on the expression of delta opioid receptor，β-arrestin 1，and Bcl-2 in rats with ulcerative colitis |
| LI Xue-jun，,YU Hong-wu，,WU Jing， |
| (The Second Affiliated Hospital of Anhui University of Chinese Medicine，Hefei 230061，Anhui，China) |
| Abstract: |
| Objective：To investigate the effect of Piwei Peiyuan enema prescription on the expression of delta opioid receptor（DOR)，β-arrestin 1，and Bcl-2 in colon tissue in rats with ulcerative colitis（UC).Methods：A total of 120 healthy male Sprague-Dawley rats were randomly divided into normal group，model group，low-，medium-，and high-dose Piwei Peiyuan enema prescription groups，and mesalazine group，with 20 rats in each group.All rats except those in the normal group were treated with 2，4，6-trinitrobenzene sulfonic acid/ethanol to establish a rat model of UC.The rats in the normal group and the model group were given enema with 0.9% sodium chloride injection，those in the mesalazine group were given enema with mesalazine suspension at a dose of 3mL/day，and those in the high-，medium-，and low-dose Piwei Peiyuan enema prescription groups were given enema with Piwei Peiyuan enema prescription 3mL/day at concentrations of crude drug of 0.56，0.28，and 0.14 g/L，respectively；the course of treatment was 15 consecutive days for all groups，and the rats were sacrificed on day 16.Immunohistochemistry and real-time PCR were used to measure the number of DOR/β-arrestin 1/Bcl-2-immunoreactive cells and the mRNA expression of DOR，β-arrestin 1，and Bcl-2.Results：Compared with the normal group，the model group had significant increases in the number of DOR/β-arrestin 1/Bcl-2-immunoreactive cells and the mRNA expression of DOR，β-arrestin 1，and Bcl-2 in colon tissue（P<0.05).Compared with the model group，the high-，medium-，and low-dose Piwei Peiyuan enema prescription groups and the mesalazine group had significant reductions in the number of DOR/β-arrestin 1/Bcl-2-immunoreactive cells and the mRNA expression of DOR，β-arrestin 1，and Bcl-2 in colon tissue（P<0.05).Conclusion：Piwei Peiyuan enema prescription exerts a therapeutic effect on rats with UC by affecting the expression of DOR，β-arrestin 1，and Bcl-2. |
| Key words: ulcerative colitis rat delta opioid receptor β-arrestin 1 Bcl-2 Piwei Peiyuan enema prescription experimental study |
