| 摘要: |
| 目的：通过检测大鼠胃癌前病变（Precancerous Lesion of Gastric Cancer，PLGC）胃黏膜微环境P13k/Akt/mTOR通路的相关指标，揭示半夏泻心汤对PLGC大鼠胃黏膜微环境的影响及防治作用机制。方法：将50只清洁级雄性SD大鼠采用改良MNNG+复合法造模，分别于造模第10周末和第20周末各随机抽取5只检测造模是否成功。将剩余40只模型大鼠随机分为4组：中药组（予半夏泻心汤灌胃）、西药组（予叶酸片液灌胃）、中西药结合组（予半夏泻心汤+叶酸片液灌胃）和模型对照组（继续造模处理），每组10只，于药物干预的第6周和第12周末停药2d后各组随机抽取5只实验大鼠观察药物干预对PLGC大鼠胃黏膜微环境的影响。结果：药物干预第6周末不成模，故无结果。干预第12周末，与模型对照组比较，中药组大鼠胃黏膜P13k和P53表达高，Akt、mTOR、HIF-1α和Bcl-2表达低，差异均有统计学意义（P<0.05）。结论：半夏泻心汤可使PLGC大鼠胃黏膜微环境P13k的表达升高，Akt、mTOR的表达降低，从而使HIF-1α的表达水平降低，由此提高P53水平，降低Bcl-2的表达，达到治疗甚至逆转PLGC的目的。 |
| 关键词: PLGC 半夏泻心汤 P13k/Akt/mTOR 实验研究 |
| DOI： |
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| Effect of Banxia Xiexin decoction on the P13k/Akt/mTOR pathway in gastric mucosa of rats with precancerous lesion of gastric cancer and related preventive and treatment methods |
| PENG Xue-jing，,LIU Jie |
| (Tianjin University of Traditional Chinese Medicine，Tianjin 300193，China;The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine，Tianjin 300150，China) |
| Abstract: |
| Objective：To investigate the indices of the P13k/Akt/mTOR pathway in gastric mucosa of rats with precancerous lesion of gastric cancer（PLGC)，as well as the effect of Banxia Xiexin decoction on the gastric mucosa of PLGC rats and its mechanism of action in prevention and treatment.Methods：A total of 50 clean male Sprague-Dawley rats were selected and the modified MNNG method was used to establish a rat model.At the end of weeks 10 and 20 of modeling，5 rats each were randomly selected to evaluate whether the model was successfully established.The remaining 40 rats were randomly divided into traditional Chinese medicine（TCM）group（treated with Banxia Xiexin decoction by gavage)，Western medicine group（treated with folic acid solution by gavage)，TCM-Western medicine group（treated with Banxia Xiexin decoction+folic acid solution by gavage)，and model control group，with 10 rats in each group.At the end of weeks 6 and 12 of drug intervention，5 rats were randomly selected from each group on day 2 after drug withdrawal to observe the effect of drug intervention on the microenvironment of gastric mucosa in PLGC rats.Results：The model was not successfully established at week 6 of drug intervention.At the end of week 12 of drug intervention，compared with the model control group，the TCM group had significantly higher expression of P13k and P53 and significantly lower expression of Akt，mTOR，HIF-1α，and Bcl-2 in gastric mucosa（P<0.05).Conclusion：Banxia Xiexin decoction can increase the expression of P13k and reduce the expression of Akt and mTOR in the gastric mucosa of PLGC rats，thus reducing the expression of HIF-1α，increasing the level of P53，and reducing the expression of Bcl-2.Banxia Xiexin decoction can help with the treatment or even reversal of PLGC. |
| Key words: precancerous lesion of gastric cancer Banxia Xiexin decoction P13k/Akt/mTOR experimental study |
