| 摘要: |
| 目的：研究复方柴芩退热颗粒对脂多糖介导炎症细胞模型的作用。方法：建立脂多糖介导RAW264.7细胞炎症模型，分为空白组，模型组，复方柴芩退热颗粒高、中、低浓度组，分别使用复方柴芩退热颗粒高、中、低剂量（21.12、10.56、5.28mg/mL）进行药物干预培养。酶联免疫法检测细胞培养上清液肿瘤坏死因子（TNF）-α、白介素（IL）-1β、IL-6及IL-10的含量。蛋白印迹法检测核转录因子（NF）-κB P-IκB表达水平。结果：复方柴芩退热颗粒高、中、低浓度组TNF-α、IL-1β及IL-6水平均显著低于模型组，差异有统计学意义（P<0.05），且呈浓度依赖性降低；IL-10水平均显著高于模型组，差异有统计学意义（P<0.05），但无浓度依赖性，其中复方柴芩退热颗粒高、中浓度组IL-10水平比较，差异无统计学意义（P>0.05）。复方柴芩退热颗粒高、中、低浓度组P-IκB表达水平均显著低于模型组，差异有统计学意义（P<0.05），且呈浓度依赖性降低。结论：复方柴芩退热颗粒具有显著抗炎作用，不仅能够显著降低LPS诱导细胞炎症模型P-IκB及TNF-α、IL-1β、IL-6的表达，同时还能升高IL-10的水平，其作用机制可能通过抑制IκB的磷酸化而阻断NF-κB信号通路有关。 |
| 关键词: 柴芩退热颗粒 核转录因子-κB 脂多糖 细胞炎症模型 |
| DOI： |
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| Effect of compound Chaiqin Tuire granules on the cell model of lipopolysaccharide-mediated inflammation based on the nuclear factor-κB pathway |
| LI Jie，,CAI Miao-shan，,LI Zhi-yong |
| (Guangdong Second Provincial Traditional Chinese Medicine Hospital，Guangzhou 510095，Guangdong，China) |
| Abstract: |
| Objective：To investigate the effect of compound Chaiqin Tuire granules on the cell model of lipopolysaccharide-mediated inflammation.Methods：RAW264.7 cells were used to establish a model of lipopolysaccharide-mediated inflammation.The cells were divided into blank group，model group，and high-，medium-，and low-concentration compound Chaiqin Tuire granule groups，which were cultured with high-，medium-，and low-dose compound Chaiqin Tuire granules（21.12，10.56，and 5.28 mg/ml，respectively).ELISA was used to measure the content of tumor necrosis factor-α（TNF-α)，interleukin-1β（IL-1β)，interleukin-6（IL-6)，and inleukin-10（IL-10) in cell supernatant，and Western blot was used to measure the expression of nuclear factor-κB（NF-κB) P-IκB.Results：Compared with the model group，the high-，medium-，and low-concentration compound Chaiqin Tuire granule groups had significantly lower levels of TNF-α，IL-1β，and IL-6（P<0.05) with reductions in a concentration-dependent manner and a significantly higher level of IL-10（P<0.05) without concentration dependence，while there was no significant difference in the level of IL-10 between the high- and medium-concentration compound Chaiqin Tuire granule groups（P>0.05).The high-，medium-，and low-concentration compound Chaiqin Tuire granule groups had significantly lower expression of P-IκB than the model group（P<0.05) with reductions in a concentration-dependent manner.Conclusion：Compound Chaiqin Tuire granules have a marked anti-inflammatory effect and can significantly reduce the expression of P-IκB，TNF-α，IL-1β，and IL-6 and increase the level of IL-10 in a cell model of lipopolysaccharide-induced inflammation，possibly by inhibiting the phosphorylation of P-IκB and blocking the NF-κB signaling pathway. |
| Key words: Chaiqin Tuire granule nuclear factor-κB lipopolysaccharide cell inflammation model |
