| 摘要: |
| 目的：优选大黄总蒽醌的提取工艺。方法：以大黄游离总蒽醌为主要指标，考察闪式法、超声法与回流法3种不同提取方法对其含量的影响，并采用正交实验法优化闪式提取法的工艺。结果：闪式法与回流法对大黄游离总蒽醌的提取率分别为87.1%、87.0%，均高于超声提取法的84.3%；闪式法每次提取时间为3min，较回流法与超声法更为迅速高效。闪式提取法优选的工艺条件为：料液比（g/mL）1∶9，提取时间80s，转速4000r/min。结论：闪式提取法能避免大黄蒽醌苷受热水解，提取时间短、效率高、经济节能，具有工业化生产应用的前景。 |
| 关键词: 大黄总蒽醌 闪式提取法 正交实验 |
| DOI： |
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| A process study on flash extraction for total anthraquinones in Rheum officinale |
| LUO Li-xia，,YI Wei，,TANG Huai-bo |
| (Xiangtan Municipal Hospital of Traditional Chinese Medicine，Xiangtan 411100，Hunan，China;Xiangtan University，Xiangtan 411105，Hunan，China) |
| Abstract: |
| Objective：To investigate the optimal extraction process for total anthraquinones in Rheum officinale.Methods：The content of total dissociative anthraquinones in Rheum officinale was used as the main index to investigate the influence of flash extraction，ultrasonic extraction，and refluxing extraction on the content of total anthraquinones，and an orthogonal experiment was performed to optimize the process of flash extraction.Results：Flash extraction and refluxing extraction had a higher extraction rate of total dissociative anthraquinones than ultrasonic extraction（87.1%/87.0%vs84.3%).The time of flash extraction was 3 minutes，which was significantly shorter than that of refluxing extraction and ultrasonic extraction.The optimal process of flash extraction was a solid-liquid ratio（g/ml）of 1∶9，an extraction time of 80 seconds，and a rotating speed of 4000r/min.Conclusion：Flash extraction can avoid the hydrolysis of anthraquinone glycoside due to heat in Rheum officinale and has a short extraction time，high efficiency，and low economic and energy costs.Therefore，it holds promise for application in industrial production. |
| Key words: Total anthraquinones in Rheum officinale flash extraction orthogonal experiment |
