| 摘要: |
| 目的：观察肾气丸对肾阳虚证大鼠肝细胞果糖6磷酸激酶（PFK）、乳酸脱氢酶（LDH）、丙酮酸激酶（PK）以及已糖激酶（HK）活性的影响。方法：于60只SD大鼠中随机选取48只，采用氢化可的松腹腔注射的方法制备肾阳虚证大鼠模型，随机分为模型组、肾气丸组、肾气丸去附桂组、附子桂枝组，每组12只，分别予相应药物灌胃；剩余12只设为正常组，予等体积0.9%氯化钠注射液灌胃。采用ELISA法分别测定各组PFK、LDH、PK、HK的活性。结果：与正常组比较，模型组PFK、LDH、PK、HK活性均降低，差异具有统计学意义（P<0.01）；与模型组比较，肾气丸组上述指标活性均明显增高，差异具有统计学意义（P<0.05，P<0.01）；与肾气丸组比较，桂枝附子组、肾气丸去附桂组上述指标活性均明显降低，差异具有统计学意义（P<0.05，P<0.01）。结论：肾气丸能明显提高肾阳虚证大鼠肝细胞内PFK、LDH、PK、HK活性，这些酶活性的上调使得线粒体内合成能量的来源增加，提高机体的能量代谢，这在一定程度上表明肾气丸与肾阳虚证具有系统生物学上的相关性。 |
| 关键词: 肾阳虚证 肾气丸 能量代谢 PFK LDH PK HK 实验研究 |
| DOI： |
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| Mechanism of the heat-reducing and qi-generating effect of Shenqi pills from the perspective of energy metabolism and formula-syndrome association |
| QIU Lin，,ZHAO Qun-ju，,LI Mei-hong |
| (Hunan University of Chinese Medicine，Changsha 410208，Hunan，China) |
| Abstract: |
| Objective：To investigate the effect of Shenqi pills on the activities of 6-phosphofructokinase（PFK)，lactate dehydrogenase（LDH)，pyruvate kinase（PK)，and hexokinase（HK）in hepatocytes in rats with kidney-yang deficiency.Methods：A total of 60 Sprague-Dawley rats were selected，and among these rats，48 were selected to establish a rat model of kidney-yang deficiency by intraperitoneal injection of hydrocortisone and then randomly divided into model group，Shenqi pill group，modified Shenqi pill group（treated with Shenqi pills without Radix Aconiti Lateralis Preparata and Ramulus Cinnamomi)，and Radix Aconiti Lateralis Preparata-Ramulus Cinnamomi group，with 12 rats in each group，and these groups were treated with the corresponding drug by gavage；the remaining 12 rats were established as normal group and were given an equal volume of 0.9% sodium chloride injection by gavage.ELISA was used to measure the activities of PFK，LDH，PK，and HK.Results：Compared with the normal group，the model group had significant reductions in the activities of PFK，LDH，PK，and HK（P<0.01).Compared with the model group，the Shenqi pill group had significant increases in the activities of PFK，LDH，PK，and HK（P<0.05 or P<0.01).Compared with the Shenqi pill group，the Radix Aconiti Lateralis Preparata-Ramulus Cinnamomi group and the modified Shenqi pill group had significant reductions in the activities of PFK，LDH，PK，and HK（P<0.05 or P<0.01).Conclusion：Shenqi pills can significantly improve the activities of PFK，LDH，PK，and HK in hepatocytes in rats with kidney-yang deficiency.The upregulation of the activities of these enzymes increases the source of energy in the mitochondria and improve the body’s energy metabolism，which suggests the association between Shenqi pills and kidney-yang deficiency from the perspective of systems biology. |
| Key words: kidney-yang deficiency Shenqi pill energy metabolism 6-phosphofructokinase lactate dehydrogenase pyruvate kinase hexokinase experimental study |
